质粒可能参与微球菌属对马拉硫磷和毒死蜱的降解过程。

Possible involvement of plasmids in degradation of malathion and chlorpyriphos by Micrococcus sp.

作者信息

Guha A, Kumari B, Bora T C, Roy M K

机构信息

Biochemistry Division, Regional Research Laboratory, Jorhat, Assam, India.

出版信息

Folia Microbiol (Praha). 1997;42(6):574-6. doi: 10.1007/BF02815468.

DOI:10.1007/BF02815468

PMID:9508553

Abstract

Two plasmid-harboring strains of Micrococcus sp. (M-36 and AG-43) degrade malathion and chlorpyriphos. Derivatives of the strains (SDS-36 and AO-43) treated with acridine orange and sodium dodecyl sulfate could not utilize malathion and chlorpyriphos for growth as the sole carbon source. Agarose gel electrophoresis of cell extracts of M-36 and AG-43 revealed the presence of a plasmid which was absent in SDS-36 and AO-43--suggesting probable involvement of plasmids in the degradation of malathion and chlorpyriphos by M-36 and AG-43. Nalidixic acid resistance in M-36 was also lost upon elimination of plasmids.

摘要

两株携带质粒的微球菌（M-36和AG-43）能够降解马拉硫磷和毒死蜱。用吖啶橙和十二烷基硫酸钠处理后的菌株衍生物（SDS-36和AO-43）不能利用马拉硫磷和毒死蜱作为唯一碳源进行生长。对M-36和AG-43的细胞提取物进行琼脂糖凝胶电泳，结果显示存在一种质粒，而SDS-36和AO-43中不存在该质粒，这表明质粒可能参与了M-36和AG-43对马拉硫磷和毒死蜱的降解过程。在质粒消除后，M-36对萘啶酸的抗性也丧失了。