Kanazawa A, Tozuka A, Kato S, Mikami T, Abe J, Shimamoto Y
Laboratory of Plant Genetics and Evolution, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo 060, Japan.
Curr Genet. 1998 Mar;33(3):188-98. doi: 10.1007/s002940050326.
Mitochondrial DNA (mtDNA) fragments that contain cox2 and atp6 were cloned from a wild soybean (Glycine soja, accession 'B09002') and from a cultivated soybean (G. max, 'Harosoy'). Comparison of these DNAs revealed that two sets of repeated sequences, namely, 299 bp and 23 bp, were present in the 5' regions of cox2 and atp6. The 299-bp and 23-bp repeats were present close to each other on the 5' flanking region and the 5' part of the coding region of cox2 in both 'Harosoy' and 'B09002', as well as on the 5' flanking region of atp6 in 'Harosoy', while these two repeats were separated by a 706-bp nucleotide sequence that contained a truncated sequence of nad3 at the 5' flanking region of atp6 in 'B09002'. The mtDNA configurations upstream from atp6 and cox2 found in 'Harosoy' appeared to have been generated from configurations of cox2 and atp6 found in 'B09002' via recombination across the 299-bp or 23-bp repeated sequences, or vice versa, in the mitochondrial genome of the hypothetical progenitor of these plants. The 299-bp sequence was found to be interspersed in the mitochondrial genome. Eight loci were identified that include mtDNA configurations that are inter-convertible with each other via recombination across this sequence in 'B09002'. Various loci on the mitochondrial genomes of higher plants that harbor segments of the 299-bp repeats in Glycine were identified.
从野生大豆（野大豆，种质‘B09002’）和栽培大豆（大豆，‘哈罗索伊’）中克隆了包含cox2和atp6的线粒体DNA（mtDNA）片段。对这些DNA的比较显示，cox2和atp6的5'区域存在两组重复序列，即299 bp和23 bp。在‘哈罗索伊’和‘B09002’中，299 bp和23 bp的重复序列在cox2的5'侧翼区域和编码区域的5'部分彼此靠近，在‘哈罗索伊’的atp6的5'侧翼区域也如此，而在‘B09002’中，这两个重复序列在atp6的5'侧翼区域被一个706 bp的核苷酸序列隔开，该序列包含nad3的一个截短序列。在‘哈罗索伊’中发现的atp6和cox2上游的mtDNA构型似乎是通过在这些植物的假设祖先的线粒体基因组中跨越299 bp或23 bp重复序列的重组，从‘B09002’中发现的cox2和atp6的构型产生的，反之亦然。发现299 bp序列散布在线粒体基因组中。在‘B09002’中鉴定出八个位点，包括通过跨越该序列的重组可相互转化的mtDNA构型。在大豆中含有299 bp重复片段的高等植物线粒体基因组上鉴定出了各种位点。
