Amin F, Stroe A E, Kahn T, Knott P J, Kahn R S, Davidson M
Houston VAMC, Texas, USA.
Neuropsychopharmacology. 1998 Apr;18(4):317-20. doi: 10.1016/S0893-133X(97)00142-5.
To identify and control renal factors affecting plasma homovanillic acid (HVA), a dopamine metabolite and an indicator of brain dopamine activity in clinical research, nine healthy subjects were studied on 5 nonconsecutive days. First study day was the baseline and on the other days base, salt, water, or probenecid loads were given. On each day serial concentrations of HVA and serotonin metabolite 5-hyroxyindoleacetic acid (HIAA), another organic anion, in plasma were measured. Results suggested that base, salt, and water loads did not affect plasma concentrations of either metabolite. Probenecid, which partially blocks renal organic anion transport, induced similar increases in plasma HVA and HIAA. When plasma HVA:HIAA ratio was used to control for the effect of probenecid, differences between baseline and probenecid days were no longer significant. Results suggest that HVA and HIAA are similarly handled by the kidney and that simultaneously measured plasma HIAA could be used to distinguish renal influences in plasma HVA studies.
为了识别和控制影响血浆高香草酸（HVA）的肾脏因素，HVA是一种多巴胺代谢产物，也是临床研究中脑多巴胺活性的指标，对9名健康受试者进行了为期5个非连续日的研究。第一个研究日为基线，在其他日子给予碱、盐、水或丙磺舒负荷。每天测量血浆中HVA和5-羟吲哚乙酸（HIAA，另一种有机阴离子）这两种血清素代谢产物的系列浓度。结果表明，碱、盐和水负荷均不影响两种代谢产物的血浆浓度。部分阻断肾脏有机阴离子转运的丙磺舒，可使血浆HVA和HIAA产生相似程度的升高。当用血浆HVA:HIAA比值来控制丙磺舒的影响时，基线日和丙磺舒日之间的差异不再显著。结果表明，肾脏对HVA和HIAA的处理方式相似，同时测量血浆HIAA可用于区分血浆HVA研究中的肾脏影响因素。
