Frendo J L, Delage-Mourroux R, Cohen R, Pichaud F, Pidoux E, Guliana J M, Jullienne A
Institut National de la Santé et de la Recherche Médicale, U.349, Hôpital Lariboisière, Centre Viggio Petersen, Paris, France.
Thyroid. 1998 Feb;8(2):141-7. doi: 10.1089/thy.1998.8.141.
We recently reported the presence of a truncated form (h-CTR2) of the human calcitonin receptor (CTR) in TT cells, a cell line derived from medullary thyroid carcinoma (MTC). This form (h-CTR2), characterized by the absence of 16 amino acids in the first intracellular domain, was also detected in two cases of MTC. In the present study we determined the expression of CTR mRNA in a larger sample, representative of the different clinical forms of MTC, and in normal thyroid. h-CTR2 was expressed in all MTC specimens (both sporadic and familial) and in the normal thyroid samples. The expression of the receptor mRNA was higher in MTC compared with normal thyroid. Moreover, CT and CTR mRNA levels were modified significantly during proliferation. This result suggests that CT may be involved in proliferation of MTC via autocrine/paracrine regulation. Calcitonin secretion by MTC may play a role in the development and spread of these tumors.
我们最近报道，在源自甲状腺髓样癌（MTC）的TT细胞系中存在人降钙素受体（CTR）的截短形式（h-CTR2）。在两例MTC中也检测到了这种形式（h-CTR2），其特征是在第一个细胞内结构域中缺少16个氨基酸。在本研究中，我们测定了CTR mRNA在更大样本中的表达情况，该样本代表了MTC的不同临床形式以及正常甲状腺组织。h-CTR2在所有MTC标本（散发性和家族性）以及正常甲状腺样本中均有表达。与正常甲状腺相比，MTC中受体mRNA的表达更高。此外，在增殖过程中，CT和CTR mRNA水平发生了显著变化。这一结果表明，CT可能通过自分泌/旁分泌调节参与MTC的增殖。MTC分泌的降钙素可能在这些肿瘤的发生和扩散中起作用。
