Barker S L, Kopelman R, Meyer T E, Cusanovich M A
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
Anal Chem. 1998 Mar 1;70(5):971-6. doi: 10.1021/ac970706k.
Fiber-optic biosensors that are selective for nitric oxide and do not respond to most potential interferents have been prepared with cytochromes c'. Both micro- and nanosensors have been prepared, and their response is fast (< 1 s), reversible, and linear up to 1 mM nitric oxide. The detection limit is 20 microM, making the sensor useful for some biological samples, such as the macrophages studied here. While sensors have been prepared based on the fluorescence of the cytochromes c', optodes with greatly enhanced signal-to-noise ratios have been made by labeling the cytochrome c' with a fluorescent dye. Comparisons of cytochromes c' from three species of bacteria as well as of two matrixes were performed and the optimum sensor configuration is described.
已经利用细胞色素c'制备了对一氧化氮具有选择性且对大多数潜在干扰物无响应的光纤生物传感器。已经制备了微型和纳米传感器，它们的响应速度快（<1秒）、可逆，并且在高达1 mM一氧化氮的浓度范围内呈线性。检测限为20 microM，这使得该传感器可用于一些生物样品，例如此处研究的巨噬细胞。虽然已经基于细胞色素c'的荧光制备了传感器，但通过用荧光染料标记细胞色素c'，制备出了信噪比大大提高的光极。对三种细菌的细胞色素c'以及两种基质进行了比较，并描述了最佳传感器配置。
