Kruse C, Emmrich J, Rumpel E, Klinger M H, Grünweller A, Rohwedel J, Krammer H J, Kühnel W, Müller P K
Department of Medical Molecular Biology, Medical University of Lübeck, Germany.
Exp Cell Res. 1998 Feb 25;239(1):111-8. doi: 10.1006/excr.1997.3891.
Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close association of vigilin with the rough endoplasmic reticulum in rat pancreatic cells. Histological examination of these cells furthermore demonstrates the highest intensity of vigilin staining in the perinuclear, intranuclear, and basolateral regions where the endoplasmic reticulum is mainly amassed. In vivo challenge of starving rats fed prior to sacrifice raised in parallel the protein levels of both trypsin and vigilin when compared to unchallenged animals and was associated with enhanced expression of the vigilin gene. In contrast, in human and rat cell lines of pancreatic tumors with a constitutively high expression of vigilin no further stimulation by cholecystokinin treatment could be achieved. Our data provide circumstantial evidence that vigilin may play a crucial role in the ability of an organ, e.g., pancreas, to cope with the physiological demand to upregulate protein synthesis.
维吉林是一种具有连续14个KH基序的蛋白质，它是包含tRNA的多蛋白复合物的一部分。几条证据表明，在那些积极参与蛋白质合成的细胞中，维吉林的表达会增强。因此，我们在此通过免疫电子显微镜显示，在大鼠胰腺细胞中，维吉林与糙面内质网紧密相关。对这些细胞的组织学检查进一步表明，在内质网主要聚集的核周、核内和基底外侧区域，维吉林染色强度最高。与未受刺激的动物相比，在处死前喂食的饥饿大鼠的体内刺激同时提高了胰蛋白酶和维吉林的蛋白质水平，并且这与维吉林基因表达的增强有关。相反，在维吉林组成性高表达的人及大鼠胰腺肿瘤细胞系中，胆囊收缩素处理无法实现进一步刺激。我们的数据提供了间接证据，表明维吉林可能在器官（例如胰腺）应对上调蛋白质合成的生理需求的能力中起关键作用。
