ISRm4-1和ISRm9，来自苜蓿中华根瘤菌的两个新型插入序列。

ISRm4-1 and ISRm9, two novel insertion sequences from Sinorhizobium meliloti.

作者信息

Zekri S, Soto M J, Toro N

机构信息

Departamento de Microbiología del Suelo y Sistemas Simbióticos, Consejo Superior de Investigaciones Científicas, Granada, Spain.

出版信息

Gene. 1998 Jan 19;207(1):93-6. doi: 10.1016/s0378-1119(97)00625-2.

DOI:10.1016/s0378-1119(97)00625-2

PMID:9511748

Abstract

Two novel insertion sequences, ISRm4-1 and ISRm9 have been identified in Sinorhizobium meliloti. ISRm4-1 is 936-bp in length, flanked by 17-bp putative terminal inverted repeats and a putative target duplication of 3-bp. ISRm4-1 is a member of the IS5 family of insertion sequences, closely related to ISRm4. ISRm9 is 2797-bp in length and carries 25-bp inverted repeats with target duplication of 7-bp: ISRm9 belongs to the IS21 family of insertion elements. On the non-pSym plasmid pRmeGR4b from S. meliloti strain GR4, a copy of ISRm4-1 is interrupted at nucleotide 150 from its 5'-end by a copy of ISRm9. Whereas ISRm4-like elements are widespread in S. meliloti, the distribution of ISRm9 appears to be correlated to that of pRmeGR4b-type plasmids.

摘要

在苜蓿中华根瘤菌中鉴定出了两个新的插入序列，即ISRm4-1和ISRm9。ISRm4-1长度为936 bp，两侧是17 bp的推定末端反向重复序列和一个3 bp的推定靶标重复序列。ISRm4-1是插入序列IS5家族的成员，与ISRm4密切相关。ISRm9长度为2797 bp，带有25 bp的反向重复序列，靶标重复序列为7 bp：ISRm9属于插入元件IS21家族。在苜蓿中华根瘤菌GR4菌株的非共生质粒pRmeGR4b上，ISRm4-1的一个拷贝在其5'端第150个核苷酸处被ISRm9的一个拷贝中断。虽然ISRm4样元件在苜蓿中华根瘤菌中广泛存在，但ISRm9的分布似乎与pRmeGR4b型质粒的分布相关。