编码肿瘤坏死因子受体相关因子5（TRAF5）人类同源物的cDNA的克隆与鉴定

Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).

作者信息

Mizushima S, Fujita M, Ishida T, Azuma S, Kato K, Hirai M, Otsuka M, Yamamoto T, Inoue J

机构信息

Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd, Tokyo, Japan.

出版信息

Gene. 1998 Jan 30;207(2):135-40. doi: 10.1016/s0378-1119(97)00616-1.

DOI:10.1016/s0378-1119(97)00616-1

PMID:9511754

Abstract

A cDNA encoding the human homolog of the tumor necrosis factor receptor-associated factor 5 (TRAF5) protein has been molecularly cloned from a cDNA library of Human Daudi B cell line. The sequence analysis revealed that the cDNA encoded a protein of 557 aa residues with a calculated molecular weight of 64,236. The encoded protein has typical structural characteristics shown in the TRAF family of proteins and binds to the cytoplasmic region of lymphotoxin-beta receptor more efficiently than to that of CD40 and CD30. The TRAF5 gene was mapped to the human chromosome 1q32.3-q41.1. Overexpression of human TRAF5 activates NF kappa B transcription factor in human 293T kidney cells. These results suggest that the human TRAF5 protein could be involved in the signal triggered by various members of the tumor necrosis factor receptor (TNFR) superfamily including CD40, CD30 and lymphotoxin-beta receptor.

摘要

从人Daudi B细胞系的cDNA文库中分子克隆出一种编码肿瘤坏死因子受体相关因子5（TRAF5）蛋白人类同源物的cDNA。序列分析显示，该cDNA编码一个由557个氨基酸残基组成的蛋白质，计算分子量为64236。所编码的蛋白质具有TRAF蛋白家族中显示的典型结构特征，并且与淋巴毒素-β受体的胞质区域结合比与CD40和CD30的胞质区域结合更有效。TRAF5基因定位于人类染色体1q32.3-q41.1。人TRAF5的过表达在人293T肾细胞中激活NF-κB转录因子。这些结果表明，人TRAF5蛋白可能参与由肿瘤坏死因子受体（TNFR）超家族的各种成员（包括CD40、CD30和淋巴毒素-β受体）触发的信号传导。