Ruiz P, Mailhot S, Delgado P, Amador A, Viciana A L, Ferrer L, Zacharievich N
Department of Pathology, University of Miami School of Medicine, Florida 33101, USA.
Cytometry. 1998 Feb 15;34(1):30-5. doi: 10.1002/(sici)1097-0320(19980215)34:1<30::aid-cyto5>3.0.co;2-i.
The transmembrane serine aminopeptidase dipeptidyl peptidase IV (DPP IV) (also known as CD26) participates in several immunological functions and has a binding affinity for several molecules, including collagen, which may be an integral mechanism for T cells to traverse endothelial barriers. Since CD26 is phenotypically expressed in certain T-cell malignancies, this study utilized a novel four-color cytofluorographic procedure to measure DPP IV enzymatic activity concurrently with the expression of other surface markers in an aggressive hepatosplenic T-cell lymphoma. Immunophenotypic analysis by flow cytometry revealed the tumor to be CD2+, CD3+, CD5-, CD7+, TcR-gamma/delta+, CD4-, CD8+/-, CD56+, and CD11c+. The CD26 molecule was also expressed, and DPP IV activity was present, with the maximal activity detectable after 10 min of incubation. These results represent the initial description of enzymatically active CD26 in a T-cell malignancy, and raise the possibility that this molecule may be a participant in the pathogenetic mechanisms utilized by the neoplastic cells.
跨膜丝氨酸氨基肽酶二肽基肽酶IV（DPP IV）（也称为CD26）参与多种免疫功能，并且对包括胶原蛋白在内的多种分子具有结合亲和力，这可能是T细胞穿越内皮屏障的一种完整机制。由于CD26在某些T细胞恶性肿瘤中呈表型表达，本研究采用一种新型的四色细胞荧光成像方法，在侵袭性肝脾T细胞淋巴瘤中同时测量DPP IV酶活性以及其他表面标志物的表达。通过流式细胞术进行的免疫表型分析显示，肿瘤细胞为CD2 +、CD3 +、CD5 -、CD7 +、TcR-γ/δ +、CD4 -、CD8 + / -、CD56 +和CD11c +。CD26分子也有表达，并且存在DPP IV活性，孵育10分钟后可检测到最大活性。这些结果首次描述了T细胞恶性肿瘤中具有酶活性的CD26，并增加了该分子可能参与肿瘤细胞所利用的致病机制的可能性。
