Giel-Pietraszuk M, Barciszewska M Z, Mucha P, Rekowski P, Kupryszewski G, Barciszewski J
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
Acta Biochim Pol. 1997;44(3):591-600.
New data are presented on the interaction of model synthetic peptides containing an arginine-rich region of human immunodeficiency virus (HIV-Tat), with native RNA molecules: tRNA(Phe) of Saccharomyces cerevisiae and 5S rRNA from Lupinus luteus. Both RNA species form complexes with the Tat1 (GRKKRRQRRRA) and Tat2 (GRKKRRQRRRAPQDSQTHQASLSKQPA) peptides, as shown by electrophoretic gel shift and RNase footprint assays, and CD measurements. The nucleotide sequence UGGG located in the dihydrouridine loop of tRNAPhe as well as in the loop D of 5S rRNA is specifically protected against RNases. Our data indicate direct interactions of guanine of RNA moieties with arginine residues. These interactions seem similar to those observed in DNA-protein complexes, but different from those previously observed in the TAR RNA-Tat complexes.
本文展示了关于含有人类免疫缺陷病毒（HIV-Tat）富含精氨酸区域的模型合成肽与天然RNA分子相互作用的新数据：酿酒酵母的tRNA（Phe）和羽扇豆的5S rRNA。电泳凝胶迁移和核糖核酸酶足迹分析以及圆二色测量表明，这两种RNA都与Tat1（GRKKRRQRRRA）和Tat2（GRKKRRQRRRAPQDSQTHQASLSKQPA）肽形成复合物。位于tRNAPhe二氢尿嘧啶环以及5S rRNA环D中的核苷酸序列UGGG受到核糖核酸酶的特异性保护。我们的数据表明RNA部分的鸟嘌呤与精氨酸残基直接相互作用。这些相互作用似乎与在DNA-蛋白质复合物中观察到的相互作用相似，但与先前在TAR RNA-Tat复合物中观察到的值不同。
