Melone M A, Galderisi U, Iacomino G, Cipollaro M, Di Bernardo G, Cotrufo R, Peluso G, Cascino A
2nd Division of Neurology, 2nd University of Naples, School of Medicine, Italy.
Antisense Nucleic Acid Drug Dev. 1998 Feb;8(1):25-33. doi: 10.1089/oli.1.1998.8.25.
By describing the behavior of myotonin mRNA levels, from the quiescent to the differentiated state in C2 mouse myoblasts, we produced evidence bearing on the role of myotonin gene product in the control of cell growth and differentiation. To study the role of myotonin in myotonic dystrophy (DM) pathogenesis, we developed a suitable cellular model where myotonin gene expression was modulated by phosphorothioate antisense oligonucleotides in C2 cultured cells. Furthermore, an isoform of the gene product, similar to that described in humans and not yet described in the mouse, was found.
通过描述肌强直性营养不良蛋白（myotonin）mRNA水平在C2小鼠成肌细胞从静止状态到分化状态的变化行为，我们获得了关于肌强直性营养不良蛋白基因产物在细胞生长和分化控制中作用的证据。为了研究肌强直性营养不良蛋白在强直性肌营养不良（DM）发病机制中的作用，我们建立了一个合适的细胞模型，在该模型中，C2培养细胞中肌强直性营养不良蛋白基因的表达受到硫代磷酸酯反义寡核苷酸的调控。此外，还发现了一种基因产物的异构体，类似于在人类中描述的但尚未在小鼠中描述的异构体。
