多嘧啶序列结合蛋白功能结构域的确定
Determination of functional domains in polypyrimidine-tract-binding protein.
作者信息
Oh Y L, Hahm B, Kim Y K, Lee H K, Lee J W, Song O, Tsukiyama-Kohara K, Kohara M, Nomoto A, Jang S K
机构信息
Department of Life Science, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang, Kyungbuk 790-784, South Korea.
出版信息
Biochem J. 1998 Apr 1;331 ( Pt 1)(Pt 1):169-75. doi: 10.1042/bj3310169.
Abstract
Polypyrimidine-tract-binding protein (PTB) is involved in pre-mRNA splicing and internal-ribosomal-entry-site-dependent translation. The biochemical properties of various segments of PTB were analysed in order to understand the molecular basis of the PTB functions. The protein exists in oligomeric as well as monomeric form. The central part of PTB (amino acids 169-293) plays a major role in the oligomerization. PTB contains several RNA-binding motifs. Among them, the C-terminal part of PTB (amino acids 329-530) exhibited the strongest RNA-binding activity. The N-terminal part of PTB is responsible for the enhancement of RNA binding by HeLa cell cytoplasmic factor(s).
摘要
多嘧啶序列结合蛋白（PTB）参与前体mRNA剪接和内部核糖体进入位点依赖性翻译。为了理解PTB功能的分子基础，对PTB不同片段的生化特性进行了分析。该蛋白以寡聚体和单体形式存在。PTB的中央部分（氨基酸169 - 293）在寡聚化中起主要作用。PTB包含多个RNA结合基序。其中，PTB的C末端部分（氨基酸329 - 530）表现出最强的RNA结合活性。PTB的N末端部分负责HeLa细胞质因子增强RNA结合。
相似文献
1
Determination of functional domains in polypyrimidine-tract-binding protein.多嘧啶序列结合蛋白功能结构域的确定
Biochem J. 1998 Apr 1;331 ( Pt 1)(Pt 1):169-75. doi: 10.1042/bj3310169.
2
3
4
Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein.RNA与聚嘧啶序列结合蛋白相互作用的化学位移图谱
Nucleic Acids Res. 2002 Jan 15;30(2):456-62. doi: 10.1093/nar/30.2.456.
5
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.由多嘧啶序列结合蛋白的组织特异性同源物诱导的hnRNP复合物的协同组装。
Mol Cell Biol. 2000 Oct;20(20):7463-79. doi: 10.1128/MCB.20.20.7463-7479.2000.
6
Nucleocytoplasmic shuttling of polypyrimidine tract-binding protein is uncoupled from RNA export.多嘧啶序列结合蛋白的核质穿梭与RNA输出解偶联。
Mol Biol Cell. 2001 Dec;12(12):3808-20. doi: 10.1091/mbc.12.12.3808.
7
Mutations in RRM4 uncouple the splicing repression and RNA-binding activities of polypyrimidine tract binding protein.RRM4中的突变使多嘧啶序列结合蛋白的剪接抑制和RNA结合活性解偶联。
RNA. 2002 Feb;8(2):137-49. doi: 10.1017/s1355838202015029.
8
Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein.多个RNA识别基序有助于RNA与多嘧啶序列结合蛋白的结合特异性和亲和力。
Biochemistry. 1997 Sep 30;36(39):11881-90. doi: 10.1021/bi9711745.
9
10
Murine polypyrimidine tract binding protein. Purification, cloning, and mapping of the RNA binding domain.小鼠多嘧啶序列结合蛋白。RNA结合结构域的纯化、克隆及定位
J Biol Chem. 1991 Dec 25;266(36):24657-63.
引用本文的文献
1
RNA Binding Protein PTBP1 Promotes the Metastasis of Gastric Cancer by Stabilizing mRNA.RNA 结合蛋白 PTBP1 通过稳定 mRNA 促进胃癌的转移。
Cells. 2024 Jan 12;13(2):140. doi: 10.3390/cells13020140.
2
Reviewing PTBP1 Domain Modularity in the Pre-Genomic Era: A Foundation to Guide the Next Generation of Exploring PTBP1 Structure-Function Relationships.回顾前基因组时代的 PTBP1 结构域模块性：指导探索 PTBP1 结构-功能关系的下一代研究的基础。
Int J Mol Sci. 2023 Jul 7;24(13):11218. doi: 10.3390/ijms241311218.
3
De-dimerization of PTB is catalyzed by PDI and is involved in the regulation of p53 translation.PTB 的解聚由 PDI 催化，并参与 p53 翻译的调节。
Nucleic Acids Res. 2021 Sep 20;49(16):9342-9352. doi: 10.1093/nar/gkab708.
4
Translational Control of the Picornavirus Phenotype.微小核糖核酸病毒表型的翻译控制
Mol Biol. 2001;35(4):591-599. doi: 10.1023/A:1010531228348.
5
Trans-acting translational regulatory RNA binding proteins.反式作用翻译调控 RNA 结合蛋白。
Wiley Interdiscip Rev RNA. 2018 May;9(3):e1465. doi: 10.1002/wrna.1465. Epub 2018 Jan 17.
6
Post-Transcriptional Regulation of Endothelial Nitric Oxide Synthase Expression by Polypyrimidine Tract-Binding Protein 1.多嘧啶序列结合蛋白1对内皮型一氧化氮合酶表达的转录后调控
Arterioscler Thromb Vasc Biol. 2015 Oct;35(10):2153-60. doi: 10.1161/ATVBAHA.115.305750. Epub 2015 Aug 20.
7
Picornaviruses and nuclear functions: targeting a cellular compartment distinct from the replication site of a positive-strand RNA virus.微小核糖核酸病毒与核功能：靶向一个不同于正链RNA病毒复制位点的细胞区室。
Front Microbiol. 2015 Jun 18;6:594. doi: 10.3389/fmicb.2015.00594. eCollection 2015.
8
Impact of PTBP1 rs11085226 on glucose-stimulated insulin release in adult Danes.PTBP1基因rs11085226对成年丹麦人葡萄糖刺激胰岛素释放的影响。
BMC Med Genet. 2015 Mar 20;16:17. doi: 10.1186/s12881-015-0160-7.
9
Rapid degradation of replication-dependent histone mRNAs largely occurs on mRNAs bound by nuclear cap-binding proteins 80 and 20.核帽结合蛋白 80 和 20 结合的 mRNA 上，依赖复制的组蛋白 mRNA 迅速降解。
Nucleic Acids Res. 2013 Jan;41(2):1307-18. doi: 10.1093/nar/gks1196. Epub 2012 Dec 11.
10
Neuronal regulation of pre-mRNA splicing by polypyrimidine tract binding proteins, PTBP1 and PTBP2.多聚嘧啶 tract 结合蛋白 PTBP1 和 PTBP2 对前体 mRNA 剪接的神经元调控。
Crit Rev Biochem Mol Biol. 2012 Jul-Aug;47(4):360-78. doi: 10.3109/10409238.2012.691456. Epub 2012 Jun 2.
本文引用的文献
1
Signal sequences that target nuclear import and nuclear export of pre-mRNA-binding proteins.靶向前体mRNA结合蛋白核输入和核输出的信号序列。
Cold Spring Harb Symp Quant Biol. 1995;60:663-8. doi: 10.1101/sqb.1995.060.01.071.
2
3
A cytoplasmic 57-kDa protein that is required for translation of picornavirus RNA by internal ribosomal entry is identical to the nuclear pyrimidine tract-binding protein.一种通过内部核糖体进入进行微小核糖核酸病毒RNA翻译所必需的57千道尔顿细胞质蛋白，与核嘧啶序列结合蛋白相同。
Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7642-6. doi: 10.1073/pnas.90.16.7642.
4
Interaction of polypyrimidine tract binding protein with the encephalomyocarditis virus mRNA internal ribosomal entry site.多嘧啶序列结合蛋白与脑心肌炎病毒mRNA内部核糖体进入位点的相互作用。
Biochemistry. 1993 Aug 17;32(32):8268-75. doi: 10.1021/bi00083a030.
5
The cellular polypeptide p57 (pyrimidine tract-binding protein) binds to multiple sites in the poliovirus 5' nontranslated region.细胞多肽p57（嘧啶序列结合蛋白）与脊髓灰质炎病毒5'非翻译区的多个位点结合。
J Virol. 1994 Feb;68(2):941-50. doi: 10.1128/JVI.68.2.941-950.1994.
6
The translation initiation factor eIF-4B contains an RNA-binding region that is distinct and independent from its ribonucleoprotein consensus sequence.翻译起始因子eIF-4B包含一个与核糖核蛋白共有序列不同且独立的RNA结合区域。
Mol Cell Biol. 1994 Apr;14(4):2307-16. doi: 10.1128/mcb.14.4.2307-2316.1994.
7
The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by Transformer 2.特定蛋白质-RNA和蛋白质-蛋白质相互作用在Transformer 2对前体mRNA剪接的正负调控中的作用。
Cell. 1994 Feb 25;76(4):735-46. doi: 10.1016/0092-8674(94)90512-6.
8
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.高等真核生物多嘧啶序列结合蛋白的不同结合特异性和功能
Science. 1995 May 26;268(5214):1173-6. doi: 10.1126/science.7761834.
9
Regulation of alternative 3' splice site selection by constitutive splicing factors.组成型剪接因子对可变3'剪接位点选择的调控。
RNA. 1995 May;1(3):234-45.
10
Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.用纯化的T7 RNA聚合酶合成传染性脊髓灰质炎病毒RNA
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2330-4. doi: 10.1073/pnas.83.8.2330.
Zotero 插件