Newton L A, Chilton N B, Beveridge I, Gasser R B
Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
Int J Parasitol. 1998 Feb;28(2):331-6. doi: 10.1016/s0020-7519(97)00149-5.
Sequences of the second internal transcribed spacer (ITS-2) of ribosomal DNA were determined for the trichostrongylid nematodes Cooperia surnabada and Cooperia oncophora, to test the hypothesis that they represent one species. Also included for comparison were other morphologically distinct species within the genus, namely Cooperia punctata and Cooperia curticei. There were no differences in the consensus ITS-2 sequences between C. oncophora and C. surnabada, whereas each taxon differed from C. punctata and C. curticei by 1.7% and 4.1%, respectively. Also, C. punctata differed from C. curticei by 5.0%. Based on these results and the DNA studies of other trichostrongylid species, it is proposed that C. oncophora and C. surnabada represent a single species.
测定了毛圆科线虫苏氏古柏线虫和牛古柏线虫核糖体DNA第二内部转录间隔区（ITS-2）的序列，以检验它们代表同一个物种的假设。为作比较，还纳入了该属内其他形态上有明显差异的物种，即点状古柏线虫和柯氏古柏线虫。牛古柏线虫和苏氏古柏线虫的ITS-2共有序列没有差异，而每个分类单元与点状古柏线虫和柯氏古柏线虫的差异分别为1.7%和4.1%。此外，点状古柏线虫与柯氏古柏线虫的差异为5.0%。基于这些结果以及对其他毛圆科物种的DNA研究，有人提出牛古柏线虫和苏氏古柏线虫代表同一个物种。
