Bachem S, Faires N, Stülke J
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany.
FEMS Microbiol Lett. 1997 Nov 15;156(2):233-8. doi: 10.1111/j.1574-6968.1997.tb12733.x.
Bacillus subtilis utilizes glucose as the preferred source of carbon and energy. Glucose is transported and concomitantly phosphorylated by the glucose permease (PtsG) of the phosphoenolpyruvate:sugar phosphotransferase system. The phosphate is transferred from enzyme I via HPr and domains IIA and IIB of the glucose permease to the sugar. In this study mutants affected in the putative phosphorylation sites of glucose permease were constructed and the effect on sugar transport and glucose repression tested. Phosphorylation of both domains IIAGlc and IIBGlc is required for efficient glucose transport and repression of beta-xylosidase and the bglPH operon.
枯草芽孢杆菌利用葡萄糖作为首选的碳源和能源。葡萄糖通过磷酸烯醇丙酮酸：糖磷酸转移酶系统的葡萄糖通透酶（PtsG）进行转运并同时磷酸化。磷酸从酶I经由HPr以及葡萄糖通透酶的IIA和IIB结构域转移至糖上。在本研究中，构建了在葡萄糖通透酶假定磷酸化位点发生突变的突变体，并测试了其对糖转运和葡萄糖阻遏的影响。高效的葡萄糖转运以及β-木糖苷酶和bglPH操纵子的阻遏需要IIAGlc和IIBGlc两个结构域的磷酸化。
