基质金属蛋白酶抑制剂对铜绿假单胞菌蛋白酶的影响

[The effect of matrix metalloproteinase inhibitor on pseudomonal proteinases].

作者信息

Kosaku K, Maeda N, Yokoyama T, Inoue T, Yoshida Y, Hosokawa T, Sawa M

机构信息

Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 1998 Feb;102(2):83-7.

PMID:9513352

Abstract

We examined the effect of CS-610, a newly developed matrix metalloproteinase (MMP) inhibitor, on pseudomonal proteinase in vitro. Alkaline proteinase (1143-4977 unit/ml Type I collagenase equivalent) and elastase (13.6-22.6 unit/ml Type I collagenase equivalent) were obtained from strains of P. aeruginosa of IID-1117, IID-1030 and IID-1130. Zymographic analysis of cultured broth of P. aeruginosa demonstrated that CS-610 inhibited alkaline proteinase with an IC50 (50% inhibition concentration) of 1.06-29.0 (x 10(-8)M) and elastase with an IC50 of 1.0-33.3 (x 10(-8)M). CS-610 is a potent inhibitor of pseudomonal proteinases.

摘要

我们在体外研究了新开发的基质金属蛋白酶（MMP）抑制剂CS - 610对铜绿假单胞菌蛋白酶的作用。碱性蛋白酶（1143 - 4977单位/毫升I型胶原酶当量）和弹性蛋白酶（13.6 - 22.6单位/毫升I型胶原酶当量）分别从IID - 1117、IID - 1030和IID - 1130的铜绿假单胞菌菌株中获得。铜绿假单胞菌培养液的酶谱分析表明，CS - 610抑制碱性蛋白酶的IC50（50%抑制浓度）为1.06 - 29.0（×10⁻⁸M），抑制弹性蛋白酶的IC50为1.0 - 33.3（×10⁻⁸M）。CS - 610是铜绿假单胞菌蛋白酶的有效抑制剂。