Mewies M, McIntire W S, Scrutton N S
Department of Biochemistry, University of Leicester, UK.
Protein Sci. 1998 Jan;7(1):7-20. doi: 10.1002/pro.5560070102.
The first identified covalent flavoprotein, a component of mammalian succinate dehydrogenase, was reported 42 years ago. Since that time, more than 20 covalent flavoenzymes have been described, each possessing one of five modes of FAD or FMN linkage to protein. Despite the early identification of covalent flavoproteins, the mechanisms of covalent bond formation and the roles of the covalent links are only recently being appreciated. The main focus of this review is, therefore, one of mechanism and function, in addition to surveying the types of linkage observed and the methods employed for their identification. Case studies are presented for a variety of covalent flavoenzymes, from which general findings are beginning to emerge.
42年前报道了首个被鉴定出的共价黄素蛋白，它是哺乳动物琥珀酸脱氢酶的一个组成部分。从那时起，已描述了20多种共价黄素酶，每种酶都具有FAD或FMN与蛋白质连接的五种模式之一。尽管共价黄素蛋白很早就被鉴定出来了，但共价键形成的机制以及共价连接的作用直到最近才被认识到。因此，本综述的主要重点除了概述所观察到的连接类型及其鉴定方法外，还包括机制和功能方面。文中给出了多种共价黄素酶的案例研究，从中开始浮现出一些一般性的发现。
