Martin G E, Myers R D
Pharmacol Biochem Behav. 1976 May;4(5):551-60. doi: 10.1016/0091-3057(76)90197-0.
To determine whether endogenous dopamine (DA) is involved in the control of feeding and drinking, the cerebral activity of 14C-DA was examined in the rat while the animal consumed food or water. A push-pull guide tube was implanted above sites either adjacent to the third ventricle, the anterior hypothalamus or the substantia nigra in each of 41 rats. After the endogenous stores of DA at specific sites were labelled by a microinjection of 0.5 to 2.0 muCi of 14C-DA, an artificial CSF was perfused at half-hour intervals at a rate of 20-23 mul/min in these sites in the food deprived rat. After a 14C-washout curve of radioactivity in the perfusate was derived for successive control samples, the food-deprived rat was offered food or water which was ingested during the course of one or more perfusions. As the rat consumed food, 14C-DA was released in some experiments from circumscribed sites in the nucleus reuniens and the zona incerta. The efflux of 14C-DA from certain sites in the circumscribed sites in the nucleus reuniens and the zona incerta. The efflux of 14C-DA from certain sites in the ventromedial and dorsomedial hypothalamus as well as from the substantia nigra also was enhanced as the rat depressed a lever to obtain food pellets. Since 14C-DA was also released from the zona incerta, perifornical hypothalamus, and into the third ventricle as the rat drank water, these results suggest that dopaminergic neurons in the brain stem play some part in the motor component of ingestive behavior rather than feeding per se.
为了确定内源性多巴胺（DA）是否参与摄食和饮水的调控，在大鼠进食或饮水时检测了其脑内14C - DA的活性。在41只大鼠的每只鼠脑第三脑室附近、下丘脑前部或黑质等部位上方植入了推挽式引导管。在特定部位通过微量注射0.5至2.0微居里的14C - DA标记内源性DA储备后，对禁食的大鼠以20 - 23微升/分钟的速率每隔半小时在这些部位灌注人工脑脊液。在获得连续对照样本的灌注液放射性的14C洗脱曲线后，给禁食大鼠提供食物或水，大鼠在一次或多次灌注过程中摄取食物或水。在某些实验中，当大鼠进食时，14C - DA从 reuniens核和未定带的特定部位释放。14C - DA从reuniens核和未定带特定部位的某些位点流出。当大鼠按压杠杆获取食丸时，腹内侧和背内侧下丘脑以及黑质某些部位的14C - DA流出也增强。由于当大鼠饮水时14C - DA也从未定带、穹窿周下丘脑释放并进入第三脑室，这些结果表明脑干中的多巴胺能神经元在摄食行为的运动成分而非摄食本身中发挥了一定作用。
