一种针对植物减数分裂细胞联会复合体相关染色质的荧光原位杂交方法。
A method for fluorescence in situ hybridization against synaptonemal complex-associated chromatin of plant meiocytes.
作者信息
Cuñado N, Santos J L
机构信息
Departamento de Genética, Facultad de Biología, Universidad Complutense, Madrid, Spain.
出版信息
Exp Cell Res. 1998 Feb 25;239(1):179-82. doi: 10.1006/excr.1997.3892.
PMID:9514796
Abstract
An improved method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescent in situ hybridization is described. This technique produces clear preparations of SCs and, in addition, consistently reveals the organization and location of different repetitive DNA sequences in plant meiotic prophase chromosomes.
摘要
本文描述了一种改进的制备与荧光原位杂交相关的植物联会复合体（SCs）二维表面铺展的方法。该技术能产生清晰的SCs制剂，此外，还能持续揭示植物减数分裂前期染色体中不同重复DNA序列的组织和位置。
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