Hoffmann R, Abdel'Al S, Engels P
Novartis Pharma, Basel, Switzerland.
Cell Biochem Biophys. 1998;28(2-3):103-13. doi: 10.1007/BF02737807.
Currently not much is known about the distribution and function of the phosphodiesterase type 7 (PDE-7) enzyme. Therefore, we carried out an extensive distribution analysis of the rat and human PDE-7 by in situ hybridization as well as RT-PCR. We isolated a partial rat cDNA clone that is highly homologous to the sequence of the human PDE-7 gene. RT-PCR tissue distribution analyses revealed expression of the mRNA of the human and rat-enzymes in most of the examined tissues, like adult heart, lung, brain, and liver, as well as in several cell lines of the immune system. In situ hybridization with the rat PDE-7 showed a differential expression pattern during the late phases of the developing rat brain with higher levels of mRNA in cortical and telencephalic structures in d 16, 18, and 20 embryonic stages, whereas in adult rat brain, higher amounts of mRNA could only be detected in cerebellum and, to a lesser extent, in hippocampus and the olfactory system.
目前，关于7型磷酸二酯酶（PDE - 7）的分布和功能了解不多。因此，我们通过原位杂交以及逆转录聚合酶链反应（RT - PCR）对大鼠和人类的PDE - 7进行了广泛的分布分析。我们分离出了一个部分大鼠cDNA克隆，它与人类PDE - 7基因的序列高度同源。RT - PCR组织分布分析显示，人类和大鼠该酶的mRNA在大多数检测组织中都有表达，如成年心脏、肺、脑和肝脏，以及免疫系统的几种细胞系。用大鼠PDE - 7进行原位杂交显示，在发育中的大鼠脑后期存在差异表达模式，在胚胎第16、18和20阶段，皮质和端脑结构中的mRNA水平较高，而在成年大鼠脑中，仅在小脑中能检测到较高量的mRNA，在海马体和嗅觉系统中的量较少。
