Tittawella I
Department of Cell and Molecular Biology, University of Umeå, Sweden.
FEBS Lett. 1998 Feb 27;423(3):351-6. doi: 10.1016/s0014-5793(98)00132-x.
Evidence presented over the years in support of mitochondrial translation in trypanosomes, based largely on studies using differential inhibitors such as cycloheximide and chloramphenicol, remains controversial. I have studied endogenous mRNA-dependent translation in a mitochondrial fraction isolated from the trypanosomatid protozoan Crithidia fasciculata. By using pancreatic ribonuclease to inactivate contaminating cytosolic activity, I show that these mitochondria can conduct protein synthesis in their own right. The mitochondrial translational products differed from cytosolic products as judged by SDS-PAGE, and had sizes expected of some proteins encoded in the mitochondrial genome of C. fasciculata and other trypanosomatids. Some evidence is provided suggesting that the seat of translation might be the kinetoplast.
多年来所呈现的支持锥虫线粒体翻译的证据仍存在争议，这些证据主要基于使用诸如环己酰亚胺和氯霉素等不同抑制剂的研究。我研究了从锥虫原生动物纤细短膜虫分离出的线粒体部分中的内源性mRNA依赖性翻译。通过使用胰核糖核酸酶使污染的胞质活性失活，我表明这些线粒体自身能够进行蛋白质合成。通过SDS-PAGE判断，线粒体翻译产物与胞质产物不同，其大小与纤细短膜虫及其他锥虫线粒体基因组中编码的一些蛋白质预期大小相符。提供了一些证据表明翻译位点可能是动质体。
