百日咳博德特氏菌-大肠杆菌杂交RNA聚合酶：启动子激活的选择性
Hybrid Bordetella pertussis-Escherichia coli RNA polymerases: selectivity of promoter activation.
作者信息
Steffen P, Ullmann A
机构信息
Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.
出版信息
J Bacteriol. 1998 Mar;180(6):1567-9. doi: 10.1128/JB.180.6.1567-1569.1998.
Abstract
We constructed hybrid Bordetella pertussis-Escherichia coli RNA polymerases and compared productive interactions between transcription activators and cognate RNA polymerase subunits in an in vitro transcription system. Virulence-associated genes of B. pertussis, in the presence of their activator BvgA, are transcribed by all variants of hybrid RNA polymerases, whereas transcription at the E. coli lac promoter regulated by the cyclic AMP-catabolite gene activator protein has an absolute requirement for the E. coli alpha subunit. This suggests that activator contact sites involve a high degree of selectivity.
摘要
我们构建了百日咳博德特氏菌-大肠杆菌杂交RNA聚合酶，并在体外转录系统中比较了转录激活因子与同源RNA聚合酶亚基之间的有效相互作用。在其激活因子BvgA存在的情况下，百日咳博德特氏菌的毒力相关基因可被杂交RNA聚合酶的所有变体转录，而由环腺苷酸-分解代谢基因激活蛋白调控的大肠杆菌乳糖启动子的转录则绝对需要大肠杆菌α亚基。这表明激活因子接触位点具有高度的选择性。
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The Bordetella pertussis sigma subunit of RNA polymerase confers enhanced expression of fha in Escherichia coli.百日咳博德特氏菌RNA聚合酶的σ亚基可增强丝状血凝素在大肠杆菌中的表达。
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