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1
Hybrid Bordetella pertussis-Escherichia coli RNA polymerases: selectivity of promoter activation.百日咳博德特氏菌-大肠杆菌杂交RNA聚合酶：启动子激活的选择性
J Bacteriol. 1998 Mar;180(6):1567-9. doi: 10.1128/JB.180.6.1567-1569.1998.
2
Nature of DNA binding and RNA polymerase interaction of the Bordetella pertussis BvgA transcriptional activator at the fha promoter.百日咳博德特氏菌BvgA转录激活因子在fha启动子处与DNA的结合性质及与RNA聚合酶的相互作用
J Bacteriol. 1997 Mar;179(5):1755-63. doi: 10.1128/jb.179.5.1755-1763.1997.
3
Synergistic binding of RNA polymerase and BvgA phosphate to the pertussis toxin promoter of Bordetella pertussis.RNA聚合酶与磷酸化的BvgA对百日咳博德特氏菌百日咳毒素启动子的协同结合。
J Bacteriol. 1995 Nov;177(22):6486-91. doi: 10.1128/jb.177.22.6486-6491.1995.
4
Differential binding of BvgA to two classes of virulence genes of Bordetella pertussis directs promoter selectivity by RNA polymerase.百日咳博德特氏菌的BvgA与两类毒力基因的差异结合通过RNA聚合酶指导启动子选择性。
Mol Microbiol. 1996 Aug;21(3):557-65. doi: 10.1111/j.1365-2958.1996.tb02564.x.
5
The modular architecture of bacterial response regulators. Insights into the activation mechanism of the BvgA transactivator of Bordetella pertussis.细菌应答调节因子的模块化结构。对百日咳博德特氏菌BvgA反式激活因子激活机制的见解。
J Mol Biol. 1994 Aug 19;241(3):363-77. doi: 10.1006/jmbi.1994.1513.
6
Phosphorylated BvgA is sufficient for transcriptional activation of virulence-regulated genes in Bordetella pertussis.磷酸化的BvgA足以激活百日咳博德特氏菌中毒力调节基因的转录。
EMBO J. 1996 Jan 2;15(1):102-9.
7
Overexpression of the RNA polymerase alpha subunit reduces transcription of Bvg-activated virulence genes in Bordetella pertussis.RNA聚合酶α亚基的过表达降低了百日咳博德特氏菌中Bvg激活的毒力基因的转录。
J Bacteriol. 2000 Jan;182(2):529-31. doi: 10.1128/JB.182.2.529-531.2000.
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Transcriptional activation of Agrobacterium tumefaciens virulence gene promoters in Escherichia coli requires the A. tumefaciens RpoA gene, encoding the alpha subunit of RNA polymerase.根癌土壤杆菌毒力基因启动子在大肠杆菌中的转录激活需要根癌土壤杆菌的RpoA基因，该基因编码RNA聚合酶的α亚基。
J Bacteriol. 1999 Aug;181(15):4533-9. doi: 10.1128/JB.181.15.4533-4539.1999.
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In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA.体内博德特氏菌毒力调控响应调节子 BvgA 的磷酸化动态。
Mol Microbiol. 2013 Apr;88(1):156-72. doi: 10.1111/mmi.12177. Epub 2013 Mar 14.
10
Identification of a Bordetella pertussis regulatory factor required for transcription of the pertussis toxin operon in Escherichia coli.鉴定大肠杆菌中百日咳毒素操纵子转录所需的百日咳博德特氏菌调节因子。
J Bacteriol. 1995 Jul;177(13):3801-7. doi: 10.1128/jb.177.13.3801-3807.1995.

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Co-evolution of RNA polymerase with RbpA in the phylum Actinobacteria.放线菌门中RNA聚合酶与RbpA的共同进化。
Appl Transl Genom. 2012 May 26;1:9-20. doi: 10.1016/j.atg.2012.03.001. eCollection 2012 Dec 1.
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Bordetella pertussis fim3 gene regulation by BvgA: phosphorylation controls the formation of inactive vs. active transcription complexes.百日咳博德特氏菌fim3基因受BvgA调控：磷酸化控制无活性与活性转录复合物的形成。
Proc Natl Acad Sci U S A. 2015 Feb 10;112(6):E526-35. doi: 10.1073/pnas.1421045112. Epub 2015 Jan 26.
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In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA.体内博德特氏菌毒力调控响应调节子 BvgA 的磷酸化动态。
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4
The Bordetella pertussis model of exquisite gene control by the global transcription factor BvgA.百日咳博德特氏菌中全局性转录因子 BvgA 对精细基因调控的模式。
Microbiology (Reading). 2012 Jul;158(Pt 7):1665-1676. doi: 10.1099/mic.0.058941-0. Epub 2012 May 24.
5
Different requirements for σ Region 4 in BvgA activation of the Bordetella pertussis promoters P(fim3) and P(fhaB).不同的 σ 区 4 需求在百日咳博德特氏菌启动子 P(fim3)和 P(fhaB)的 BvgA 激活中。
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6
Novel architectural features of Bordetella pertussis fimbrial subunit promoters and their activation by the global virulence regulator BvgA.百日咳博德特氏菌菌毛亚基启动子的新型结构特征及其被全局毒力调节因子 BvgA 的激活。
Mol Microbiol. 2010 Sep;77(5):1326-40. doi: 10.1111/j.1365-2958.2010.07293.x.
7
The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro.来自苜蓿中华根瘤菌的RNA聚合酶α亚基可与来自大肠杆菌的RNA聚合酶亚基组装，并在体内和体外的基础转录和激活转录中发挥作用。
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Reconstitution of acetosyringone-mediated Agrobacterium tumefaciens virulence gene expression in the heterologous host Escherichia coli.乙酰丁香酮介导的根癌农杆菌毒性基因在异源宿主大肠杆菌中的表达重构
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9
Transcriptional activation of Agrobacterium tumefaciens virulence gene promoters in Escherichia coli requires the A. tumefaciens RpoA gene, encoding the alpha subunit of RNA polymerase.根癌土壤杆菌毒力基因启动子在大肠杆菌中的转录激活需要根癌土壤杆菌的RpoA基因，该基因编码RNA聚合酶的α亚基。
J Bacteriol. 1999 Aug;181(15):4533-9. doi: 10.1128/JB.181.15.4533-4539.1999.

本文引用的文献

1
Characterization of DNA binding sites for the BvgA protein of Bordetella pertussis.百日咳博德特氏菌BvgA蛋白DNA结合位点的特征分析
J Bacteriol. 1997 Jun;179(11):3790-2. doi: 10.1128/jb.179.11.3790-3792.1997.
2
The Bordetella pertussis sigma subunit of RNA polymerase confers enhanced expression of fha in Escherichia coli.百日咳博德特氏菌RNA聚合酶的σ亚基可增强丝状血凝素在大肠杆菌中的表达。
Mol Microbiol. 1997 Mar;23(5):945-54. doi: 10.1046/j.1365-2958.1997.2741639.x.
3
Nature of DNA binding and RNA polymerase interaction of the Bordetella pertussis BvgA transcriptional activator at the fha promoter.百日咳博德特氏菌BvgA转录激活因子在fha启动子处与DNA的结合性质及与RNA聚合酶的相互作用
J Bacteriol. 1997 Mar;179(5):1755-63. doi: 10.1128/jb.179.5.1755-1763.1997.
4
Central role of the BvgS receiver as a phosphorylated intermediate in a complex two-component phosphorelay.BvgS受体作为复杂双组分磷酸化信号转导中磷酸化中间体的核心作用。
J Biol Chem. 1996 Dec 27;271(52):33176-80. doi: 10.1074/jbc.271.52.33176.
5
Differential binding of BvgA to two classes of virulence genes of Bordetella pertussis directs promoter selectivity by RNA polymerase.百日咳博德特氏菌的BvgA与两类毒力基因的差异结合通过RNA聚合酶指导启动子选择性。
Mol Microbiol. 1996 Aug;21(3):557-65. doi: 10.1111/j.1365-2958.1996.tb02564.x.
6
Transcription factor recognition surface on the RNA polymerase alpha subunit is involved in contact with the DNA enhancer element.RNA聚合酶α亚基上的转录因子识别表面参与与DNA增强子元件的接触。
EMBO J. 1996 Aug 15;15(16):4358-67.
7
Escherichia coli RNA polymerase holoenzyme: rapid reconstitution from recombinant alpha, beta, beta', and sigma subunits.大肠杆菌RNA聚合酶全酶：从重组的α、β、β'和σ亚基快速重组
Methods Enzymol. 1996;273:130-4. doi: 10.1016/s0076-6879(96)73012-4.
8
Phosphorylation-dependent binding of BvgA to the upstream region of the cyaA gene of Bordetella pertussis.百日咳博德特氏菌BvgA与cyaA基因上游区域的磷酸化依赖性结合
Mol Microbiol. 1996 May;20(3):489-96. doi: 10.1046/j.1365-2958.1996.5231057.x.
9
Integration of multiple domains in a two-component sensor protein: the Bordetella pertussis BvgAS phosphorelay.双组分传感蛋白中多个结构域的整合：百日咳博德特氏菌BvgAS磷酸化信号转导系统
EMBO J. 1996 Mar 1;15(5):1028-36.
10
Phosphorylated BvgA is sufficient for transcriptional activation of virulence-regulated genes in Bordetella pertussis.磷酸化的BvgA足以激活百日咳博德特氏菌中毒力调节基因的转录。
EMBO J. 1996 Jan 2;15(1):102-9.

百日咳博德特氏菌-大肠杆菌杂交RNA聚合酶：启动子激活的选择性

Hybrid Bordetella pertussis-Escherichia coli RNA polymerases: selectivity of promoter activation.

作者信息

Steffen P, Ullmann A

机构信息

Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.

出版信息

J Bacteriol. 1998 Mar;180(6):1567-9. doi: 10.1128/JB.180.6.1567-1569.1998.

DOI:10.1128/JB.180.6.1567-1569.1998
PMID:9515928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107059/
Abstract

We constructed hybrid Bordetella pertussis-Escherichia coli RNA polymerases and compared productive interactions between transcription activators and cognate RNA polymerase subunits in an in vitro transcription system. Virulence-associated genes of B. pertussis, in the presence of their activator BvgA, are transcribed by all variants of hybrid RNA polymerases, whereas transcription at the E. coli lac promoter regulated by the cyclic AMP-catabolite gene activator protein has an absolute requirement for the E. coli alpha subunit. This suggests that activator contact sites involve a high degree of selectivity.

摘要

我们构建了百日咳博德特氏菌-大肠杆菌杂交RNA聚合酶，并在体外转录系统中比较了转录激活因子与同源RNA聚合酶亚基之间的有效相互作用。在其激活因子BvgA存在的情况下，百日咳博德特氏菌的毒力相关基因可被杂交RNA聚合酶的所有变体转录，而由环腺苷酸-分解代谢基因激活蛋白调控的大肠杆菌乳糖启动子的转录则绝对需要大肠杆菌α亚基。这表明激活因子接触位点具有高度的选择性。