Cróinín T O, Clyne M, Drumm B
Department of Paediatrics, University College Dublin, The Children's Research Centre, Our Lady's Hospital for Sick Children, Dublin, Ireland.
Gastroenterology. 1998 Apr;114(4):690-6. doi: 10.1016/s0016-5085(98)70582-7.
BACKGROUND & AIMS: Molecular mimicry of Lewis blood group antigens by Helicobacter pylori may be involved in immune evasion by the bacteria and in the pathogenesis of chronic atrophic gastritis. Helicobacter mustelae infects ferrets naturally, causing gastritis, and may be involved in ulcerogenesis. The aim of this study was to determine if H. mustelae shows a similar form of molecular mimicry.
Antibodies raised against H. mustelae were used to stain ferret gastric tissue by immunoblotting, immunohistochemistry, and flow cytometry. Epitopes recognized by cross-reactivity were characterized by proteinase K and sodium metaperiodate treatment.
H. mustelae antiserum reacted with H. mustelae and with ferret gastric tissue. Absorption of the antiserum with H. mustelae or ferret and rabbit gastric tissue removed the cross-reactive antibodies. Antibodies reacted with a blood group antigen A-like structure on ferret gastric epithelial cells and H. mustelae lipopolysaccharide.
H. mustelae expresses a blood group-like antigen as part of its lipopolysaccharide that may be used as a method of immune evasion by mimicry of gastric epithelial cells. The cross-reactivity shown by H. mustelae-specific antibodies with gastric mucosa may suggest a role for autoantibodies in the pathogenesis of H. mustelae-induced gastritis in ferrets.
幽门螺杆菌对Lewis血型抗原的分子模拟可能参与细菌的免疫逃逸及慢性萎缩性胃炎的发病机制。鼬獾幽门螺杆菌自然感染雪貂，可引起胃炎，且可能参与溃疡形成。本研究旨在确定鼬獾幽门螺杆菌是否表现出类似的分子模拟形式。
用针对鼬獾幽门螺杆菌产生的抗体，通过免疫印迹、免疫组织化学和流式细胞术对雪貂胃组织进行染色。通过蛋白酶K和偏高碘酸钠处理对交叉反应识别的表位进行表征。
鼬獾幽门螺杆菌抗血清与鼬獾幽门螺杆菌及雪貂胃组织发生反应。用鼬獾幽门螺杆菌或雪貂及兔胃组织吸收抗血清可去除交叉反应抗体。抗体与雪貂胃上皮细胞上的血型抗原A样结构及鼬獾幽门螺杆菌脂多糖发生反应。
鼬獾幽门螺杆菌在其脂多糖中表达一种血型样抗原，这可能是其通过模拟胃上皮细胞进行免疫逃逸的一种方式。鼬獾幽门螺杆菌特异性抗体与胃黏膜的交叉反应可能提示自身抗体在鼬獾幽门螺杆菌诱导的雪貂胃炎发病机制中起作用。
