一种通过对聚合酶链反应扩增的16S核糖体DNA进行HhaI酶切，利用限制性片段长度多态性模式将发光杆菌属与弧菌属分离的新方法。
A new approach to separate the genus Photobacterium from Vibrio with RFLP patterns by HhaI digestion of PCR-amplified 16S rDNA.
作者信息
Urakawa H, Kita-Tsukamoto K, Ohwada K
机构信息
Ocean Research Institute, University of Tokyo, 1-15-1, Minamidai, Nakano-ku, Tokyo 164, Japan.
出版信息
Curr Microbiol. 1998 Mar;36(3):171-4. doi: 10.1007/pl00006762.
A new approach to separate members of the genus Photobacterium from the genus Vibrio with RFLP (Restriction Fragment Length Polymorphism) patterns by HhaI digestion of PCR-amplified 16S rDNA was developed in the present study. It was clearly shown that these patterns of the genus Photobacterium were unique and distinguishable from Vibrio species. This method is very simple and does not need other supporting procedures, such as Southern transfer and probe hybridization. It can be applied not only to luminous species, but also to non-luminous Photobacterium spp. This result promises a rapid tool to distinguish the genus Photobacterium from Vibrio and should be useful in routine identification system.
本研究开发了一种新方法，通过对PCR扩增的16S rDNA进行HhaI酶切，利用限制性片段长度多态性（RFLP）模式将发光杆菌属成员与弧菌属分离。结果清楚地表明，发光杆菌属的这些模式是独特的，可与弧菌属物种区分开来。该方法非常简单，不需要其他辅助程序，如Southern转移和探针杂交。它不仅可以应用于发光物种，也可以应用于非发光的发光杆菌属物种。这一结果有望成为一种快速区分发光杆菌属与弧菌属的工具，在常规鉴定系统中应会很有用。
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