咖啡潜叶蛾（Perileucoptera spp.）对苏云金芽孢杆菌δ-内毒素的敏感性：一种抗内果皮昆虫转基因多年生作物的模型。
Susceptibility of the coffee leaf miner (Perileucoptera spp.) to bacillus thuringiensis delta-endotoxins: A model for transgenic perennial crops resistant to endocarpic insects.
作者信息
Filho OG, Denolf P, Peferoen M, Decazy B, Eskes AB, Frutos R
机构信息
Secao de Genetica, Instituto Agronomico de Campinas, C.P. 28, 13100 Campinas, S.P., Brazil.
出版信息
Curr Microbiol. 1998 Mar;36(3):175-9. doi: 10.1007/pl00006763.
Binding of several Bacillus thuringiensis delta-endotoxins was studied on histological midgut sections of larvae of coffee leaf miner Perileucoptera coffeella from Brazil and Perileucoptera sp from Madagascar. CryIA(a), CryIA(b), CryIA(c), CryIB, CryIE, and CryIIA were tested for binding, and only CryIA(c), CryIB, and CryIE yielded a positive response. The toxins bound to the whole midgut, and the result was identical on both insect populations. The same toxins, to the number of which CryIC was added, were tested on larvae of P. coffeella. CryIA(c) and CryIB were toxic with an LC50 of 1.47 &mgr;g/ml and 21.93 &mgr;g/ml, respectively. CryIE was not toxic to P. coffeella. CryIA(c) and CryIB were tested for synergistic activity and were shown to act by cumulative effect when delivered to the insect larvae as a mixture.
对来自巴西的咖啡潜叶蛾（Perileucoptera coffeella）幼虫和来自马达加斯加的Perileucoptera sp幼虫的中肠组织切片进行了几种苏云金芽孢杆菌δ-内毒素结合情况的研究。测试了CryIA(a)、CryIA(b)、CryIA(c)、CryIB、CryIE和CryIIA的结合情况，只有CryIA(c)、CryIB和CryIE产生了阳性反应。毒素与整个中肠结合，并且在两种昆虫种群上的结果相同。在咖啡潜叶蛾幼虫上测试了添加了CryIC的相同毒素。CryIA(c)和CryIB具有毒性，LC50分别为1.47μg/ml和21.93μg/ml。CryIE对咖啡潜叶蛾无毒。测试了CryIA(c)和CryIB的协同活性，结果表明当作为混合物施用于昆虫幼虫时，它们通过累积效应起作用。
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