一种260 kDa神经母细胞瘤表面抗原的表达，细胞毒性天然人IgM的作用靶点：与MYCN扩增的相关性及视黄酸的影响

Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM: correlation to MYCN amplification and effects of retinoic acid.

作者信息

David K, Ehrhardt A, Ollert M W, Erttmann R, Bredehorst R, Vogel C W

机构信息

Department of Biochemistry and Molecular Biology, University of Hamburg, Germany.

出版信息

Eur J Cancer. 1997 Oct;33(12):1937-41. doi: 10.1016/s0959-8049(97)00207-4.

DOI:10.1016/s0959-8049(97)00207-4

PMID:9516828

Abstract

Human neuroblastoma cells contain a 260 kDa surface-associated antigen (NB-p260) that is recognised by natural cytotoxic IgM antibodies. In this study we demonstrate that NB-p260 is expressed in vivo in a neuroblastoma tumour specimen but not in normal human tissues of neuronal origin. Since MYCN amplification is a clinical marker of neuroblastoma disease progression, we analysed the expression of NB-p260 in human neuroblastoma cell lines with different MYCN amplification status. However, both amplified and non-amplified neuroblastoma cell lines exhibited comparable NB-p260 expression. Treatment of neuroblastoma cells with the differentiation-inducing agent retinoic acid (RA) also had no effect on the expression of NB-p260. Collectively, the data suggest that expression of NB-p260 on human neuroblastoma cells is independent of malignancy and differentiation status of neuroblastoma.

摘要

人神经母细胞瘤细胞含有一种260 kDa的表面相关抗原（NB-p260），可被天然细胞毒性IgM抗体识别。在本研究中，我们证明NB-p260在神经母细胞瘤肿瘤标本中在体内表达，但在神经元起源的正常人体组织中不表达。由于MYCN扩增是神经母细胞瘤疾病进展的临床标志物，我们分析了不同MYCN扩增状态的人神经母细胞瘤细胞系中NB-p260的表达。然而，扩增和未扩增的神经母细胞瘤细胞系均表现出相当的NB-p260表达。用分化诱导剂视黄酸（RA）处理神经母细胞瘤细胞对NB-p260的表达也没有影响。总体而言，数据表明人神经母细胞瘤细胞上NB-p260的表达与神经母细胞瘤的恶性程度和分化状态无关。