Rohde V, Sattler H P, Oehlenschläger B, Forster S, Zwergel T, Seitz G, Wullich B
Clinic of Urology and Pediatric Urology, University of the Saarland, Homburg/Saar, Germany.
Clin Cancer Res. 1998 Jan;4(1):197-202.
Using the sensitive telomeric repeat amplification protocol assay, we detected telomerase activity in 26 of 35 (74.3%) renal cell carcinomas analyzed. Subdivision of the tumors according to telomerase activity did not reveal an obvious association between the presence of telomerase activity and histomorphological stage, grade, tumor size, or DNA ploidy. Furthermore, no association was found between telomerase activity and a distinct chromosomal aberration pattern; namely, loss of genetic material on the short arm of chromosome 3. Telomerase activity was also detected in 6 of 35 (17.1%) normal corresponding renal tissue samples, which seems interesting in light of the supposed biological role of telomerase expression in carcinogenesis. Interestingly, telomerase activity was detected in three of the four (75%) kidneys bearing non-clear cell tumor types, whereas of the 31 kidneys with clear cell carcinomas, telomerase activity was found in only 3 (9.7%) normal tissue samples. In addition, the two renal angiomyolipomas and one of the two analyzed transitional cell carcinomas of the renal pelvis were telomerase negative.
采用敏感的端粒重复序列扩增法检测，我们在分析的35例肾细胞癌中的26例（74.3%）中检测到了端粒酶活性。根据端粒酶活性对肿瘤进行细分，未发现端粒酶活性的存在与组织形态学分期、分级、肿瘤大小或DNA倍体之间存在明显关联。此外，未发现端粒酶活性与特定的染色体畸变模式之间存在关联，即3号染色体短臂上的遗传物质缺失。在35例相应的正常肾组织样本中的6例（17.1%）中也检测到了端粒酶活性，鉴于端粒酶表达在致癌过程中的假定生物学作用，这似乎很有意思。有趣的是，在4例非透明细胞肿瘤类型的肾脏中的3例（75%）中检测到了端粒酶活性，而在31例透明细胞癌的肾脏中，仅在3例（9.7%）正常组织样本中发现了端粒酶活性。此外，两个肾血管平滑肌脂肪瘤以及两个分析的肾盂移行细胞癌中的一个端粒酶呈阴性。
