Lobacz B, Wolska K I
Department of Bacterial Genetics, University of Warsaw, Poland.
Acta Microbiol Pol. 1997;46(4):393-7.
Escherichia coli defects caused by the overproduction of DnaK and DnaJ proteins are presented. Induced overproduction of DnaK and DnaJ was determined by SDS-PAGE electrophoresis and immunoblots analysis. Elevated levels of both proteins are not deleterious for such cellular functions as growth, survival and cell morphology. However, instability of pJW14 plasmid (derivative of pACYC184) was observed as the result of dnaKdnaJ operon overexpression.
本文展示了由DnaK和DnaJ蛋白过量产生所导致的大肠杆菌缺陷。通过SDS-PAGE电泳和免疫印迹分析确定了DnaK和DnaJ的诱导性过量产生。这两种蛋白水平的升高对生长、存活和细胞形态等细胞功能并无有害影响。然而，观察到由于dnaKdnaJ操纵子的过表达，pJW14质粒（pACYC184的衍生物）出现了不稳定现象。
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