生长因子对中国仓鼠卵巢细胞中GTP酶激活蛋白（Ras GAP）的影响。

Effect of growth factor on GTPase-activating protein (Ras GAP) in Chinese hamster ovary cells.

作者信息

Sasa H, Nakata H, Umekage T, Namima M, Tomiyama K, Arimura S, Kobayashi M, Watanabe Y

机构信息

Department of Perinatal and Maternal Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan.

出版信息

Jpn J Pharmacol. 1998 Jan;76(1):121-4. doi: 10.1254/jjp.76.121.

DOI:10.1254/jjp.76.121

PMID:9517415

Abstract

GTPase-activating proteins (GAPs) stimulate the hydrolysis of GTP bound to small G-proteins and regulate the signal transduction pathway. Changes in the expression of p21-Ras p120-GAP induced by growth factor treatment were examined in cultured Chinese hamster ovary (CHO) and human choriocarcinoma (BeWo) cells. Expression of p120-GAP and GAP activity were measured. Fetal bovine serum induced a significant increased level of GAP in CHO cells, but did not increase GAP in BeWo cells. The results suggest that growth factors affect Ras GAP expression in CHO cells, while they do not in other cells such as BeWo cells.

摘要

GTP酶激活蛋白（GAPs）可刺激与小G蛋白结合的GTP水解，并调节信号转导通路。在培养的中国仓鼠卵巢（CHO）细胞和人绒毛膜癌（BeWo）细胞中，检测了生长因子处理诱导的p21-Ras p120-GAP表达变化。测定了p120-GAP的表达和GAP活性。胎牛血清可使CHO细胞中的GAP水平显著升高，但对BeWo细胞中的GAP没有影响。结果表明，生长因子可影响CHO细胞中Ras GAP的表达，而对BeWo细胞等其他细胞则无影响。