Thiele T E, Roitman M F, Bernstein I L
Department of Psychology, University of Washington, Seattle 98195, USA.
Behav Neurosci. 1998 Feb;112(1):193-8. doi: 10.1037//0735-7044.112.1.193.
With c-Fos immunoreactivity as a marker for neural activity, we examined whether environmental cues associated with ethanol injection influence the expression of tolerance to ethanol-induced c-Fos activation. Over 24 training days, male Long-Evans rats received ethanol injection (2.5 g/kg) in one environment and saline injection in a different environment. Relative to rats that received ethanol for the first time, ethanol-induced c-Fos expression in the paraventricular nucleus of the hypothalamus (PVN) and the locus coeruleus (LC) was significantly reduced in rats that had received multiple prior ethanol administrations. However, tolerance was partially reversed when ethanol was given in the saline-paired, rather than the ethanol-paired, environment. Results suggest that tolerance to ethanol, as indexed by c-Fos expression in the PVN and the LC, is mediated in part by Pavlovian conditioned responses to cues that predict ethanol administration.
以c-Fos免疫反应性作为神经活动的标志物，我们研究了与乙醇注射相关的环境线索是否会影响对乙醇诱导的c-Fos激活的耐受性表达。在24天的训练过程中，雄性Long-Evans大鼠在一种环境中接受乙醇注射（2.5 g/kg），在另一种不同的环境中接受生理盐水注射。相对于首次接受乙醇的大鼠，多次预先接受乙醇给药的大鼠中，乙醇诱导的下丘脑室旁核（PVN）和蓝斑（LC）中的c-Fos表达显著降低。然而，当在与生理盐水配对而非与乙醇配对的环境中给予乙醇时，耐受性部分逆转。结果表明，以PVN和LC中的c-Fos表达为指标的对乙醇的耐受性，部分是由对预测乙醇给药的线索的巴甫洛夫条件反应介导的。
