通过计算机显微光度分析对小鼠T细胞进行亚分类。
Subclassification of murine T cells by computerized microphotometric analysis.
作者信息
Olson G B, Bartels P H, Anderson R E
出版信息
Cell Biophys. 1979 Sep;1(3):243-54. doi: 10.1007/BF02783666.
Splenocytes separated by physical means and classified as T cells bay immunologic tests and computerized microphotometric analysis are differentiated into subgroups by analysis of the distribution patterns of Feulgen-positive nuclear DNA. In like fashion T cells obtained as purified preparations after separation on a nylon column, and accepted as T cells by micromorphometric analysis were subjected to further computerized morphometric analysis of nuclear DNA to form subgroups of cells. In each case, the number and composition of the detected subgroups were consistent. The classification does not appear to reflect any obvious phases of the cell cycle and is not dependent upon the sex and strain of mice from which the cells were obtained.
通过物理方法分离并经免疫学检测和计算机显微光度分析归类为T细胞的脾细胞，根据福尔根阳性核DNA的分布模式分析被分化为亚群。同样，在尼龙柱上分离后作为纯化制剂获得并经微观形态计量分析确认为T细胞的T细胞，也接受了核DNA的进一步计算机形态计量分析以形成细胞亚群。在每种情况下，检测到的亚群的数量和组成都是一致的。这种分类似乎并不反映细胞周期的任何明显阶段，也不依赖于获取细胞的小鼠的性别和品系。
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