Yu D T
J Immunol. 1975 Jul;115(1):91-3.
In the process of forming SRBC rosettes of human lymphocytes, two types were distinguished, the early and late rosettes. The former appeared immediately whereas the latter required further incubation at 4degreesC. In this study, the two types of cells were separated by gradient centrifugation and studied separately. It was found that the proportion of caps in the late rosettes was much greater than in the early ones. Treatment of the former by trypsin, AET, and neuraminidase decreased this proportion so that it resembled the late one. It was postulated that some of the differences in the early and late RFC lie in the differences in the content of membrane sulphydryl and sialic acid groups and distribution of SRBC receptors.
在人淋巴细胞形成SRBC玫瑰花结的过程中，可区分出两种类型，即早期玫瑰花结和晚期玫瑰花结。前者立即出现，而后者需要在4℃下进一步孵育。在本研究中，通过梯度离心将这两种类型的细胞分离并分别进行研究。结果发现，晚期玫瑰花结中帽状物的比例远高于早期玫瑰花结。用胰蛋白酶、AET和神经氨酸酶处理前者可降低这一比例，使其与早期玫瑰花结相似。据推测，早期和晚期玫瑰花结形成细胞（RFC）的一些差异在于膜巯基和唾液酸基团含量以及SRBC受体分布的差异。
