Deinl I, Angermaier L, Franzelius C, Machbert G
Institute of Forensic Medicine, Erlangen, Germany.
J Chromatogr B Biomed Sci Appl. 1997 Dec 19;704(1-2):251-8. doi: 10.1016/s0378-4347(97)00429-5.
A sensitive, simple and rapid method without sample pretreatment is presented for the simultaneous determination of flunitrazepam and its main metabolites (norflunitrazepam, 7-amino- and 7-acetamidoflunitrazepam) in urine. The single-step procedure is based on a column-switching technique which uses an immobilized antibody in an extraction column following concentration on a precolumn and separation on an analytical column. UV detection was performed at 254 nm. The reusability of the antibody exceeds 88 runs and a complete analysis was performed in less than 40 min. The method shows coefficients of variation below 9.9% and rates of recovery greater than 92% tested at the level of 50 ng/ml urine. The limit of detection was below 2 ng/ml urine for the four compounds.
本文提出了一种无需样品预处理的灵敏、简单且快速的方法，用于同时测定尿液中的氟硝西泮及其主要代谢物（去甲氟硝西泮、7-氨基氟硝西泮和7-乙酰氨基氟硝西泮）。该单步程序基于柱切换技术，该技术在预柱上富集并在分析柱上分离后，在萃取柱中使用固定化抗体。在254nm处进行紫外检测。抗体的可重复使用性超过88次运行，并且在不到40分钟内完成了完整分析。在50ng/ml尿液水平测试时，该方法的变异系数低于9.9%，回收率大于92%。这四种化合物的检测限均低于2ng/ml尿液。
