Wong V Y, Macagno E R
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Dev Genes Evol. 1998 Mar;208(1):51-5. doi: 10.1007/s004270050153.
Sequence analysis of a newly isolated Hirudo medicinalis cDNA containing an Antennapedia (Antp)-class homeobox suggests that the corresponding gene, Lox6, is an ortholog of the Drosophila Deformed (Dfd) gene. In situ hybridization of whole-mounted preparations shows that the major sites of Lox6 expression during embryogenesis are the central nervous system (CNS) and the peripheral sensory system. Lox6 mRNA can be detected in a subset of neurons in each ganglion from the subesophageal ganglion (RG2) to the most posterior ganglion, with the highest level of expression seen in RG3. Peripherally, Lox6 is expressed principally in the primordia of the sensillae and in the eyes. This pattern of expression of Lox6 suggests that one of its functions may be to contribute to the diversification of neuronal phenotypes.
对新分离出的含有触角足（Antp）类同源异型框的医用水蛭cDNA进行序列分析表明，相应的基因Lox6是果蝇变形（Dfd）基因的直系同源基因。整装标本的原位杂交显示，胚胎发育过程中Lox6表达的主要部位是中枢神经系统（CNS）和外周感觉系统。从咽下神经节（RG2）到最后端的神经节，每个神经节的一部分神经元中都能检测到Lox6 mRNA，其中RG3中的表达水平最高。在周围，Lox6主要在感器原基和眼睛中表达。Lox6的这种表达模式表明其功能之一可能是促进神经元表型的多样化。
