Hara M, Kaneko T, Nakamura C, Asada Y, Miyake J
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Biochim Biophys Acta. 1998 Mar 25;1363(3):199-208. doi: 10.1016/s0005-2728(98)00004-8.
Recently, we reported that a H-subunit-depleted photosynthetic reaction center (RC-H) was purified from purple nonsulfer photosynthetic bacterium Rhodopseudomonas viridis (Rps. viridis) using a strong detergent sodium alkyl ether sulfate. We compared the redox properties of a native photosynthetic reaction center (RC) and RC-H of Rps. viridis. In RC-H prepared by our method, secondary quinone (QB) was removed while primary quinone (QA) was retained. Absorption spectrum of RC-H was similar to that of RC. After reconstitution of ubiquinone 10 into QB sites, RC-H showed electron transfer activity that was the same as that for native RC. This is the first report about the redox properties of RC-H of Rps. viridis.
最近，我们报道了使用强去污剂烷基醚硫酸钠从紫色非硫光合细菌绿硫红假单胞菌（Rps. viridis）中纯化出一种H亚基缺失的光合反应中心（RC-H）。我们比较了绿硫红假单胞菌天然光合反应中心（RC）和RC-H的氧化还原特性。在我们通过该方法制备的RC-H中，二级醌（QB）被去除，而一级醌（QA）得以保留。RC-H的吸收光谱与RC的相似。在将泛醌10重构到QB位点后，RC-H显示出与天然RC相同的电子转移活性。这是关于绿硫红假单胞菌RC-H氧化还原特性的首次报道。
