Tsvetkova Ts, Sergeeva D, Grigorova D
Vet Med Nauki. 1976;13(2):100-4.
Established were the thin-layer chromatography conditions for the demonstration of the herbicide Zenkor as a pure substance and a preparation. Comparative experiments were carried out applying Thomson and Stanley's thin-layer chromatography conditions and the authors' personal ones. Most adequate proved to be the following conditions: sorbent-Kieselgel 60F 254 (Merck); solvent -- chloroform: 1,4-dioxan (9:1); developer -- ultraviolet light (authors' technique). Essential with this method is the preliminary (prior to apply the pure substances on the thin-layer chromatographic plaque) moving of the plaque in the indicated mobile phase (authors' technique). Under these thin-layer chromatography conditions work is made feasible and the method is fast and simple, achieving high sensitivity -- 0.5 mug Zenkor.
确立了用于证明除草剂赛克津作为纯物质和制剂的薄层色谱条件。采用汤姆森和斯坦利的薄层色谱条件以及作者个人的条件进行了对比实验。结果证明最适宜的条件如下：吸附剂——硅胶60F 254（默克公司）；溶剂——氯仿：1,4 -二氧六环（9:1）；显色剂——紫外线（作者的技术）。用此方法时，关键是在薄层色谱板上点样纯物质之前，先将薄层板在指定的流动相中展开（作者的技术）。在这些薄层色谱条件下，操作可行，该方法快速简便，灵敏度高——可检测出0.5微克赛克津。
