Colomar M C, Hirt B, Beard P
Swiss Institute for Experimental Cancer Research (I.S.R.E.C.), Epalinges, Switzerland.
J Gen Virol. 1998 Mar;79 ( Pt 3):581-6. doi: 10.1099/0022-1317-79-3-581.
Two strains of minute virus of mice (MVM) show different host-cell specificities. MVM(i) grows in T lymphocytes whereas MVM(p) is fibroblast-specific. By constructing recombinant viral DNAs between the genomes of the two strains, we have shown that two segments of the MVM(i) genome are required for lytic viral growth in T lymphocytic EL4 cells. One segment (iE) was found between nucleotides 1084 and 2070, in a region encoding the early viral proteins and containing mRNA splice signals and the late P39 promoter. The other (iL) was between nucleotides 3523 and 4339 in the region coding for capsid protein. The P39 promoters within the E segment from MVM(i) or MVM(p) were equally active in transfected EL4 cells. However, pE-containing MVM DNA produced more NS2 mRNA than iE-containing DNA, apparently the result of virus-strain-specific differences in the regulation of splicing.
两株小鼠微小病毒（MVM）表现出不同的宿主细胞特异性。MVM(i) 在T淋巴细胞中生长，而MVM(p) 具有成纤维细胞特异性。通过构建这两个毒株基因组之间的重组病毒DNA，我们发现MVM(i) 基因组的两个片段是病毒在T淋巴细胞EL4细胞中进行裂解性生长所必需的。一个片段（iE）位于核苷酸1084至2070之间，处于编码早期病毒蛋白的区域，包含mRNA剪接信号和晚期P39启动子。另一个（iL）位于编码衣壳蛋白区域的核苷酸3523至4339之间。来自MVM(i) 或MVM(p) 的E片段内的P39启动子在转染的EL4细胞中具有同等活性。然而，含pE的MVM DNA比含iE的DNA产生更多的NS2 mRNA，这显然是病毒毒株特异性的剪接调控差异所致。
