Axcrona K, Akerblad P, Leanderson T
Department of Cell and Molecular Biology, Lund University, Sweden.
Scand J Immunol. 1998 Mar;47(3):210-7. doi: 10.1046/j.1365-3083.1998.00295.x.
Both anti-CD40 antibodies and anti-immunoglobulin (Ig) coupled to Sepharose induced proliferation of resting B cells and suppressed lipopolysaccharide (LPS)-induced B-cell differentiation to immunoglobulin secretion at comparable levels determined with the plaque-forming assay and Ig RNA steady state levels. Anti-CD40 antibodies also increased the proliferation of B cells stimulated by T helper cells in vitro while suppressing their differentiation to Ig secretion. Further, B cells preactivated by anti-Ig, anti-CD40 or a combination of the two mitogens could be restimulated by anti-CD40 but not by anti-Ig antibodies. Phenotypic divergence of Ig and CD40 signals regarding surface expression of activation markers was observed. Restimulation of anti-Ig- or anti-CD40-prestimulated cells with anti-Ig induced apoptosis whereas apoptosis could be inhibited when cells were recultivated with anti-CD40.
与琼脂糖珠偶联的抗CD40抗体和抗免疫球蛋白（Ig）均能诱导静息B细胞增殖，并在通过噬斑形成试验和Ig RNA稳态水平测定的相当水平上抑制脂多糖（LPS）诱导的B细胞分化为免疫球蛋白分泌。抗CD40抗体还能增加体外T辅助细胞刺激的B细胞增殖，同时抑制其分化为Ig分泌。此外，由抗Ig、抗CD40或两种促细胞分裂剂组合预激活的B细胞可被抗CD40再次刺激，但不能被抗Ig抗体再次刺激。观察到Ig和CD40信号在激活标志物表面表达方面的表型差异。用抗Ig再次刺激抗Ig或抗CD40预刺激的细胞会诱导细胞凋亡，而当用抗CD40重新培养细胞时，细胞凋亡可被抑制。
