含人类DNA的酵母人工染色体中的脱氧核糖核酸酶超敏位点

DNase-hypersensitive sites in yeast artificial chromosomes containing human DNA.

作者信息

Svetlova E, Avril-Fournout N, Ira G, Deschavanne P, Filipski J

机构信息

Laboratoire de Mutagénèse, Institut J. Monod, Paris, France.

出版信息

Mol Gen Genet. 1998 Feb;257(3):292-8. doi: 10.1007/s004380050650.

DOI:10.1007/s004380050650

PMID:9520263

Abstract

We have mapped the DNase I-hypersensitive sites (HSs) in Yeast Artificial Chromosomes (YACs) containing segments of human chromosomal DNA. One of the five HSs found in a YAC carrying the beta-globin gene cluster has been localised in the region, termed HS2, that is DNase I hypersensitive in most human cells. We have also identified a class of HSs in YACs containing DNA from the q11.2 band of human chromosome 21, which are located close to, or within, segments of the chromosome that are sensitive to restriction enzymes recognizing CGCG tetranucleotides.

摘要

我们已经绘制了包含人类染色体DNA片段的酵母人工染色体（YAC）中的DNA酶I超敏位点（HSs）图谱。在携带β-珠蛋白基因簇的YAC中发现的五个HSs之一已定位在一个区域，称为HS2，该区域在大多数人类细胞中对DNA酶I高度敏感。我们还在包含人类21号染色体q11.2带DNA的YAC中鉴定出一类HSs，它们位于对识别CGCG四核苷酸的限制性酶敏感的染色体片段附近或内部。