Adnan S, Susan L, Weiss D
Kennedy-Leigh Centre for Horticultural Research, Hebrew University of Jerusalem, Rehovot, Israel.
Plant Mol Biol. 1998 Apr;36(6):935-9. doi: 10.1023/a:1005998404720.
Heat treatment of tomato fruits induces tolerance to chilling injury. We have previously shown that specific heat-shock proteins (HSPs) are expressed in heated tomato fruits after cold storage. To search for heat-induced genes that are expressed at low temperatures, a cDNA library prepared from pre-heated chilled tomato fruits was differentially screened. A novel cDNA clone, hcit2, encoding a protein of ca. 16.5 kDa, was isolated. The predicted protein contains three putative trans-membrane hydrophobic sequences, suggesting that the protein is membrane-localized. The expression of hcit2 in fruits was induced by high temperature, but not by other stresses such as low temperature, drought or anaerobic conditions, and not during fruit ripening. A high level of hcit2 transcript was found in heated fruits after 2 weeks at 2 degrees C. High temperatures also induced hcit2 expression in tomato leaves, flowers and stems. The HCIT2 protein may be involved in the acquisition of tolerance to chilling injury.
番茄果实的热处理可诱导其对冷害的耐受性。我们之前已经表明，特定的热休克蛋白（HSPs）在冷藏后的加热番茄果实中表达。为了寻找在低温下表达的热诱导基因，我们对从预热后的冷藏番茄果实中制备的cDNA文库进行了差异筛选。分离出了一个新的cDNA克隆hcit2，它编码一种约16.5 kDa的蛋白质。预测的蛋白质含有三个假定的跨膜疏水序列，这表明该蛋白质定位于膜上。hcit2在果实中的表达是由高温诱导的，而不是由其他胁迫如低温、干旱或厌氧条件诱导的，在果实成熟过程中也不表达。在2℃下放置2周后的加热果实中发现了高水平的hcit2转录本。高温也能诱导番茄叶片、花朵和茎中hcit2的表达。HCIT2蛋白可能参与了对冷害耐受性的获得。
