Szele F G, Cepko C L
Harvard Medical School, Howard Hughes Medical Institute, 200 Longwood Avenue, Boston, Massachusetts 02115, USA.
Dev Biol. 1998 Mar 15;195(2):100-13. doi: 10.1006/dbio.1997.8725.
Lineage analysis in the chick telencephalon was carried out using a library of retroviral vectors. Clones were analyzed in posthatch day 14-21 animals for the phenotype and final locations of sibling cells. Clones often contained multiple types of neurons and glia. Clones of more than four cells almost always crossed functional boundaries. They were dispersed primarily along the rostrocaudal axis or in multiple directions, e.g., along the rostrocaudal and mediolateral axes. In order to begin to understand how the final patterns of dispersion were reached, embryonic tissue was examined. Radial migration, apparently supported by radial glial cells, occurred within the proliferative zones in all clones. In contrast to the migration of cells in the mammalian telencephalon, no tangential migration within the proliferative zones was observed at any age examined. However, beginning at embryonic day 4.5, tangential migration in the mantle zone in multiple directions was observed among the majority of clones. This type of migration occurred as soon as a mantle zone became apparent. It appeared that the tangential migration was not along radial glial processes. As in the mammalian telencephalon and chick diencephalon, dispersion among clonally related cells in the chick telencephalon is frequent, is extensive, and results from tangential migration in a variety of directions.
利用逆转录病毒载体文库对鸡端脑进行谱系分析。在孵化后第14至21天的动物中分析克隆，以确定同胞细胞的表型和最终位置。克隆通常包含多种类型的神经元和神经胶质细胞。四个以上细胞的克隆几乎总是跨越功能边界。它们主要沿头尾轴或多个方向分散，例如沿头尾轴和中外侧轴。为了开始了解如何形成最终的分散模式，对胚胎组织进行了检查。在所有克隆的增殖区内均发生了明显由放射状胶质细胞支持的径向迁移。与哺乳动物端脑中细胞的迁移不同，在所检查的任何年龄，均未在增殖区内观察到切向迁移。然而，从胚胎第4.5天开始，在大多数克隆中观察到在套层区内多个方向的切向迁移。这种迁移在套层区一出现就发生。似乎切向迁移不是沿着放射状胶质细胞的突起进行的。与哺乳动物端脑和鸡间脑一样，鸡端脑中克隆相关细胞之间的分散很常见，范围广泛，并且是由多种方向的切向迁移导致的。
