Garden R W, Shippy S A, Li L, Moroz T P, Sweedler J V
Department of Chemistry and Beckman Institute, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3972-7. doi: 10.1073/pnas.95.7.3972.
By using matrix-assisted laser desorption/ionization time-of-flight MS, individual peptidergic neurons from Aplysia are assayed. A semiquantitative method is developed for comparing single-cell profiles by using spectral normalization, and peptides are localized to specific cells by mass spectrometric cell mapping. In addition to all previously identified products of the egg-laying hormone (ELH) gene, other peptides are formed from proteolytic hydrolysis of Leu-Leu residues within ELH and acidic peptide (AP). AP exhibits further processing to yield AP1-20 and AP9-27. These peptides appear to be colocalized in vesicles with ELH, transported to specific neuronal targets, and released in a Ca2+-dependent manner. A differential peptide distribution is observed at a specific target cell, and a low-frequency variation of AP, [Thr21]AP, is detected in a single animal.
通过使用基质辅助激光解吸/电离飞行时间质谱，对海兔的单个肽能神经元进行分析。开发了一种半定量方法，通过光谱归一化比较单细胞图谱，并通过质谱细胞图谱将肽定位到特定细胞。除了所有先前鉴定的产卵激素（ELH）基因产物外，其他肽由ELH和酸性肽（AP）内的Leu-Leu残基的蛋白水解形成。AP进一步加工产生AP1-20和AP9-27。这些肽似乎与ELH共定位在囊泡中，运输到特定的神经元靶点，并以Ca2+依赖的方式释放。在特定的靶细胞中观察到差异肽分布，并且在单个动物中检测到AP的低频变体[Thr21]AP。
