Controlling your losses: conditional gene silencing in mammals.

Methods are now widely used in mice, and to a lesser extent in mammalian-cell culture, for the constitutive silencing of target genes in order to assess their function. For a variety of reasons, not least because many genes are essential for viability, it is important that these methods can be adapted to allow the controlled silencing of target genes. Reviewed here are the ways in which gene-silencing methods can be combined with a growing number of genetic control systems to generate cell lines or mice that are, in effect, conditional mutants. These approaches are still being developed and promise to open up key areas of cell and animal biology to genetic analysis.