Collavin L, Buzzai M, Saccone S, Bernard L, Federico C, DellaValle G, Brancolini C, Schneider C
Laboratorio Nazionale Consorzio Interuniversitario Biotecnologie, Trieste, Italy.
Genomics. 1998 Mar 1;48(2):265-9. doi: 10.1006/geno.1997.5172.
Murine Gas2 is a microfilament-associated protein whose expression is increased at growth arrest in mammalian cells. During apoptosis, Gas2 is specifically cleaved at its C-terminus by a still unknown ICE-like protease, and the processed protein induces dramatic rearrangements in the cytoskeleton when overexpressed in several cell types. Here we report the characterization of a cDNA encoding the human homologue of Gas2, showing high conservation with the murine counterpart at the protein level. Fluorescence in situ hybridization analysis and radiation hybrid mapping localized the GAS2 gene on human chromosome 11p14.3-p15.2, in a region homologous to the gas2 region on mouse chromosome 7.
小鼠Gas2是一种与微丝相关的蛋白质，其表达在哺乳动物细胞生长停滞时增加。在细胞凋亡过程中，Gas2在其C末端被一种仍未知的类ICE蛋白酶特异性切割，当在几种细胞类型中过表达时，加工后的蛋白质会诱导细胞骨架发生显著重排。在此，我们报告了编码Gas2人类同源物的cDNA的特性，其在蛋白质水平上与小鼠对应物高度保守。荧光原位杂交分析和辐射杂种图谱将GAS2基因定位在人类染色体11p14.3-p15.2上，该区域与小鼠染色体7上的gas2区域同源。
