In vivo measurement of morphometric and hemodynamic changes in the microcirculation during angiogenesis under chronic alpha1-adrenergic blocker treatment.

The effect of chronic administration of the alpha 1 blocker prazosin on microvascular angiogenesis was studied in rabbit ear chamber to investigate the role of concomitant increase in flow-oriented wall shear stress in vascular remodeling during angiogenesis. Rabbits were treated with prazosin hydrochloride (50 mg/liter in water) orally after ear chambers were installed. The microcirculation in the chamber was observed and recorded from 4 to 23 postoperative day (POD) by an intravital videomicroscope. The total vascular area (TA) were quantified as a morphometric parameter. Changes in wall shear stress in venules (20-40 micron ID) were calculated from flow velocity, vessel diameter, and in vivo blood viscosity. TA was significantly increased in the prazosin-treated animals. The levels of shear stress, which was increased 1.43 times by prazosin on 9 POD, gradually decreased toward the control level on 13 POD and they almost coincided on 21 POD. These findings experimentally provided supporting evidence that shear stress is probably involved in the adaptive response as one of mechanical factors regulating vascular remodeling during angiogenesis.