Lei X G, Ross D A, Parks J E, Combs G F
Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
Biol Trace Elem Res. 1997 Winter;59(1-3):195-206. doi: 10.1007/BF02783245.
Phospholipid hydroperoxide glutathione peroxidase (PHGPX) is the second intracellular selenium (Se)-dependent glutathione peroxidase (GSH-Px) identified in mammals. Our objectives were to determine the effect of dietary vitamin E and Se levels on PHGPX activity expression in testis, epididymis, and seminal vesicles of pubertal maturing rats, and the relationship of PHGPX expression with testicular development and sperm quality. Forty Sprague-Dawley male weanling rats (21-d old), were initially fed for 3 wk a torula yeast basal diet (containing 0.05 mg Se/kg) supplemented with marginal levels of Se (0.1 mg/kg as Na2SeO3) and vitamin E (25 IU/kg as all-rac-alpha-tocopheryl acetate). Then, rats were fed the basal diets supplemented with 0 or 0.2 mg Se/kg and 0 or 100 IU vitamin E/kg diet during the 3-wk period of pubertal maturing. Compared with the Se-supplemented rats, those fed the Se-deficient diets retained 31, 88, 67, and 50% of Se-dependent GSH-Px activities in liver, testis, epididymis, and seminal vesicles, respectively. Testes and seminal vesicles had substantially higher (5- to 20-fold) PHGPX activity than liver. Dietary Se deficiency did not affect PHGPX activities in the reproductive tissues, but reduced PHGPX activity in liver by 28% (P < 0.0001). Dietary vitamin E supplementation did not affect PHGPX activity in liver, whereas it raised PHGPX activity in seminal vesicles by 43% (P < 0.005). Neither dietary vitamin E nor Se levels affected body weight gains, reproductive organ weights, or sperm counts and morphology. In conclusion, expression of PHGPX activity in testis and seminal vesicles was high and regulated by dietary Se and vitamin E differently from that in liver.
磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPX）是在哺乳动物中发现的第二种细胞内硒（Se）依赖性谷胱甘肽过氧化物酶（GSH-Px）。我们的目标是确定日粮维生素E和硒水平对青春期成熟大鼠睾丸、附睾和精囊PHGPX活性表达的影响，以及PHGPX表达与睾丸发育和精子质量的关系。40只斯普拉格-道利雄性断奶大鼠（21日龄），最初3周饲喂含边缘水平硒（0.1 mg/kg亚硒酸钠）和维生素E（25 IU/kg全消旋-α-生育酚醋酸酯）的产朊假丝酵母基础日粮（含0.05 mg硒/kg）。然后，在青春期成熟的3周期间，给大鼠饲喂补充0或0.2 mg硒/kg和0或100 IU维生素E/kg日粮的基础日粮。与补充硒的大鼠相比，饲喂缺硒日粮的大鼠肝脏、睾丸、附睾和精囊中硒依赖性GSH-Px活性分别保留了31%、88%、67%和50%。睾丸和精囊的PHGPX活性比肝脏高得多（5至20倍）。日粮缺硒不影响生殖组织中的PHGPX活性，但使肝脏中的PHGPX活性降低了28%（P<0.0001）。日粮补充维生素E不影响肝脏中的PHGPX活性，但使精囊中的PHGPX活性提高了43%（P<0.005）。日粮维生素E和硒水平均不影响体重增加、生殖器官重量或精子数量及形态。总之，睾丸和精囊中PHGPX活性的表达较高，且日粮硒和维生素E对其调节方式与肝脏不同。
