Kooreman K, Babbs C, Fessler J
Department of Basic Medical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907-1246, USA.
Am J Vet Res. 1998 Mar;59(3):340-6.
To evaluate and compare oxidative processes during ischemia and reperfusion of the equine large colon and jejunum.
2 groups of 6 anesthetized horses undergoing a terminal procedure.
Isolated loops of large colon and jejunum were subjected to 2 hours of ischemia, followed by 2 hours of reperfusion. Tissue specimens were taken after 105 minutes of ischemia and 10, 30, 60, and 120 minutes of reperfusion. Mesenteric arterial and venous blood samples were collected for blood gas analysis at the same times to evaluate ischemia and reoxygenation. Oxidative processes in tissues were evaluated by use of biochemical assays for malondialdehyde and conjugated dienes and by use of the manganese-diaminobenzidine histochemical technique for localized superoxide generation.
Significant quantities of malondialdehyde and conjugated dienes were detected in the jejunum after 60 and 120 minutes of reperfusion together with histochemical evidence of superoxide generation in jejunal endothelial cells and in submucosal neutrophils and eosinophils.
Oxidative processes do not appear to have an appreciable role in inducing damage in the equine large colon during reperfusion after 2 hours of ischemia. In contrast to the jejunum, reactive oxygen species are not generated in measurable quantities in the large colon subsequent to ischemia and reperfusion.
Free radical scavengers are not likely to be effective in prevention of equine colonic mucosal deterioration after ischemia.
评估和比较马的大结肠和空肠在缺血及再灌注过程中的氧化过程。
2组，每组6匹接受终末手术的麻醉马匹。
将分离出的大结肠和空肠肠袢进行2小时缺血处理，随后进行2小时再灌注。在缺血105分钟以及再灌注10、30、60和120分钟后采集组织样本。同时采集肠系膜动脉和静脉血样本进行血气分析，以评估缺血和再氧合情况。通过丙二醛和共轭二烯的生化检测以及使用锰 - 二氨基联苯胺组织化学技术检测局部超氧化物生成来评估组织中的氧化过程。
再灌注60分钟和120分钟后，空肠中检测到大量丙二醛和共轭二烯，同时有组织化学证据表明空肠内皮细胞、黏膜下中性粒细胞和嗜酸性粒细胞中有超氧化物生成。
在缺血2小时后的再灌注过程中，氧化过程似乎在诱导马的大结肠损伤方面没有显著作用。与空肠不同，缺血再灌注后大结肠中不会产生可测量量的活性氧。
自由基清除剂不太可能有效预防马缺血后结肠黏膜的恶化。
