Kobzdej M, Matuszyk J, Zioło E, Strzadała L
Laboratory of Cellular Immunology, Polish Academy of Sciences, Wrocław, Poland.
Arch Immunol Ther Exp (Warsz). 1997;45(4):307-13.
Mice with transgenic T cell receptor (TCR) recognizing H-Y male antigen developed spontaneous lymphomas originated from immature thymocytes, with the surface expression of transgenic TCR and CD4/CD8 co-receptors. During in vitro long-term culture (3 months) some lymphoma cell lines lost the surface expression of TCR and co-receptors. Interestingly, the proteins of transgenic receptor were expressed intracellularly but TCR was not detectable on the surface of the in vitro selected subline in contrast to TCR-positive parental cells maintained in vivo. TCR-negative subline has been found to be slowly growing in vivo (i.p. injection) and less tumorigenic (s.c. injection) than parental TCR positive lymphoma. It seems that the in vivo interactions of lymphoma cells with microenvironment preserve their TCR expression and endow with growth advantage, while the selected in vitro TCR-negative cells lose the tumorigenic potential.
具有识别H-Y雄性抗原的转基因T细胞受体（TCR）的小鼠发生了源自未成熟胸腺细胞的自发性淋巴瘤，伴有转基因TCR和CD4/CD8共受体的表面表达。在体外长期培养（3个月）期间，一些淋巴瘤细胞系失去了TCR和共受体的表面表达。有趣的是，转基因受体的蛋白质在细胞内表达，但与体内维持的TCR阳性亲代细胞相比，在体外选择的亚系表面未检测到TCR。已发现TCR阴性亚系在体内（腹腔注射）生长缓慢，并且比亲代TCR阳性淋巴瘤的致瘤性低（皮下注射）。似乎淋巴瘤细胞与微环境的体内相互作用保留了它们的TCR表达并赋予生长优势，而体外选择的TCR阴性细胞则失去了致瘤潜力。
