Ota A, Liu X, Fujio H, Sakato N, Ueda S
Department of Neurovirology, Osaka University, Suita, Japan.
Hybridoma. 1998 Feb;17(1):73-5. doi: 10.1089/hyb.1998.17.73.
Twenty monoclonal antibodies (MAbs) were obtained by immunizing Balb/c mice with recombinant p17 (rp17) of HIV-1. Epitope specificity of each MAb was determined using six peptides that cover the entire region of p17. We found that each MAb reacts with only one of the peptides, residues 12-29, 30-52, 53-87, and 87-115 (P12-29, P30-52, P53-87, P87-115) of p17 with the exception of one MAb. Three kinds of MAbs that recognize P30-52, P87-115, and a conformational epitope, suppressed the infectivity of HIV-1 (JMH-1) when they added in the culture of MT-4 cells infected by HIV-1 within 24 h of the infection.
用HIV-1的重组p17（rp17）免疫Balb/c小鼠，获得了20种单克隆抗体（MAb）。使用覆盖p17整个区域的六种肽来确定每种MAb的表位特异性。我们发现，除一种MAb外，每种MAb仅与p17的一种肽发生反应，这些肽分别是12-29位、30-52位、53-87位和87-115位（P12-29、P30-52、P53-87、P87-115）。三种识别P30-52、P87-115和一种构象表位的MAb，在感染HIV-1的MT-4细胞培养24小时内加入时，可抑制HIV-1（JMH-1）的感染性。
