Oguni M, Shinohara H, Asano T, Kato K, Setogawa T
Department of Ophthalmology, Shimane Medical University, Shimane, Japan.
Ophthalmic Res. 1998;30(2):84-9. doi: 10.1159/000055458.
We previously hypothesized that light stimulus at eye opening of rats on postnatal days (P) 13 or 14 has an effect on the expression of GTP-binding proteins (G(0) in the retina) because the concentration of G(0) alpha increased rapidly between P10 and P15. This hypothesis was also supported by the findings that the distribution of G(0) alpha in the retina was almost the same as that of adult rats between P10 and P15. In this study, pregnant rats were kept in a dark room after vaginal plugs were identified; they gave birth to their pups in the dark, and their pups were reared by their mothers in the dark. The postnatal rats were sacrificed at P10, P15, P18, P22, P24, P27, and P30. Their retinas were investigated immunochemically and immunohistochemically, using G(0) alpha antibody, and these results were compared with those of the rat pups reared normally. Only G(0) alpha immunoreactivity in the inner nuclear layer of rats reared in the dark room was weaker than in the controls; the distribution of G(0) alpha in the retina did not change, as compared with pups reared in normal conditions. In addition, in pups reared in the dark, G(0) alpha increased rapidly from P10 to P15. However, the concentration of G(0) alpha in the retina of rat pups reared in the dark was significantly low at P22 (p < 0.01) and P30 (p < 0.05), as compared with pups reared in normal conditions. Although the function of G(0) in the retina may have something to do with the light stimulus after eye opening, it appeared that the expression of G(0) was not influenced by light stimulus at eye opening.
我们之前曾假设，出生后第13或14天大鼠睁眼时的光刺激会对视网膜中GTP结合蛋白（G(0)）的表达产生影响，因为G(0)α的浓度在出生后第10天到第15天之间迅速增加。这一假设还得到了以下发现的支持：出生后第10天到第15天之间，视网膜中G(0)α的分布与成年大鼠几乎相同。在本研究中，确定出现阴道栓后，将怀孕大鼠置于暗室中；它们在黑暗中分娩，幼崽由母亲在黑暗中饲养。出生后的大鼠在出生后第10天、第15天、第18天、第22天、第24天、第27天和第30天被处死。使用G(0)α抗体对其视网膜进行免疫化学和免疫组织化学研究，并将这些结果与正常饲养的幼鼠的结果进行比较。仅在暗室中饲养的大鼠内核层中的G(0)α免疫反应性比对照组弱；与正常条件下饲养的幼崽相比，视网膜中G(0)α的分布没有变化。此外，在黑暗中饲养的幼崽中，G(0)α从出生后第10天到第15天迅速增加。然而，与正常条件下饲养的幼崽相比，在黑暗中饲养的幼鼠视网膜中G(0)α的浓度在出生后第22天（p < 0.01）和第30天（p < 0.05）时显著较低。尽管视网膜中G(0)的功能可能与睁眼后的光刺激有关，但似乎G(0)的表达不受睁眼时光刺激的影响。
