摇椅型电压门控钾通道基因在豚鼠中的表达

Expression of Shaker-type voltage-gated potassium channel genes in the guinea-pig.

作者信息

Manabe I, Tsuboi M, Ahmmed G U, Sasaki N, Ohtahara A, Yamamoto Y, Hiroe K, Yoshida A, Hisatome I, Shigemasa C

机构信息

First Department of Internal Medicine, Tottori University, Yonago, Japan.

出版信息

Res Commun Mol Pathol Pharmacol. 1998 Jan;99(1):33-40.

PMID:9523353

Abstract

Few potassium channel genes have been isolated in the guinea-pig despite detailed electrophysiological characterization of potassium channels in the guinea-pig heart. We obtained partial clones of Shaker-type potassium channel genes in the guinea-pig and demonstrated their tissue distribution. Partial clones of the Shaker-type potassium channel genes were obtained by RT-PCR or genomic PCR. mRNA expression was measured by RNase protection assays in the heart, brain, and skeletal muscle. Three of the five obtained channel genes were expressed in the guinea-pig heart; Kv1.2, Kv1.3, and Kv1.6. Kv 1.6 expression was markedly at a higher level in the atrium than in the ventricle. Expression of the channel genes in the guinea-pig was different from that in human and rat, which may contribute to the species-specific action potential waveform.

摘要

尽管对豚鼠心脏中的钾通道进行了详细的电生理学特征分析，但在豚鼠中分离出的钾通道基因却很少。我们获得了豚鼠中Shaker型钾通道基因的部分克隆，并展示了它们的组织分布。通过逆转录聚合酶链反应（RT-PCR）或基因组聚合酶链反应（genomic PCR）获得了Shaker型钾通道基因的部分克隆。通过核糖核酸酶保护分析（RNase protection assays）测量心脏、大脑和骨骼肌中的信使核糖核酸（mRNA）表达。所获得的五个通道基因中有三个在豚鼠心脏中表达；即Kv1.2、Kv1.3和Kv1.6。Kv1.6在心房中的表达水平明显高于心室。豚鼠中通道基因的表达与人类和大鼠不同，这可能导致物种特异性的动作电位波形。